[引用][C] 原核融合表达载体的设计, 构建及应用

程琰, 毛旭虎, 王庆旭, 余抒, 刘艳青, 张晓丽, 宁亚蕾… - 免疫学杂志, 2008

Development and characterization of a fully functional small anti-HER2 antibody

J Gao, B Li, H Li, X Zhang, D Zhang, L Zhao, C Wang… - BMB …, 2009 - koreascience.kr
The penetrating of monoclonal antibodies (mAbs) into solid tumor may be hampered by their
large size. The antibody mimetics, composed of two complementarity-determining regions …

[HTML][HTML] Expression and purification of SfaXII, a protein involved in regulating adhesion and motility genes in extraintestinal pathogenic Escherichia coli

P Paracuellos, A Öhman, AE Sauer-Eriksson… - Protein expression and …, 2012 - Elsevier
Pathogenic Escherichia coli strains commonly harbor genes involved in formation of
fimbriae, such as the sfaII fimbrial gene cluster found in uropathogenic and newborn …

Functional improvement of human cardiotrophin 1 produced in tobacco chloroplasts by co-expression with plastid thioredoxin m

M Ancín, R Sanz-Barrio, E Santamaría… - Plants, 2020 - mdpi.com
Human cardiotrophin 1 (CT1), a cytokine with excellent therapeutic potential, was previously
expressed in tobacco chloroplasts. However, the growth conditions required to reach the …

[图书][B] Medicinal protein engineering

YE Khudyakov - 2008 - taylorfrancis.com
An All-Inclusive Review of the Achievements and Trends in the Fast-Growing Protein
Engineering Field From humble beginnings like making fire for mere survival, engineering …

[PDF][PDF] 血管内皮生长因子单链抗体在大肠埃希菌中的高效表达

张冀原, 陈羽, 林涵, 何晴, 徐威 - 微生物学杂志, 2019 - wswx.cnjournals.com
摘要以血管内皮生长因子单链抗体(pTA-anti-VEGF-scFv) 为研究对象, 考察不同培养基配方和
培养条件对血管内皮生长因子单链抗体表达量的影响. 经均匀设计试验, 确定工程菌R22-4 …

Co-expression for intracellular processing in microbial protein production

Q Lu, JC Aon - Biotechnology letters, 2014 - Springer
The biological activity of a recombinant protein is highly dependent on its biophysical
properties including post-translational modifications, solubility, and stability. Production of …

Влияние коэкспрессии тиоредоксина и прохимозина на рефолдинг рекомбинантного химозина альпака

СВ Беленькая, ДН Щербаков… - … журнал генетики и …, 2023 - vavilov.elpub.ru
Аннотация Молокосвертывающий фермент химозин является представителем группы
аспартатных протеиназ. Химозин–основной компонент сычужного фермента …

Exploring Protein Function and Stability: Case Studies on Cryoprotection and Thermal Stability of Antifreeze Proteins, Enzyme Inhibition of SARS-Cov-2 Main …

K Jovic - 2023 - search.proquest.com
In this work, I have explored basic questions related to protein structure, function, and
stability, which are critical for understanding the fundamental mechanisms of biology. The …

In vivo drafting of single‐chain antibodies for regulatory duty on the σ54‐promoter Pu of the TOL plasmid

P Jurado, LA Fernández… - Molecular …, 2006 - Wiley Online Library
The identification of single‐chain antibodies (scFvs) that interfere in vivo with the building of
the complex that activate the prokaryotic, σ54‐dependent promoter Pu of the catabolic TOL …