Improving the Thermostability of Xylanase A from Bacillus subtilis by Combining Bioinformatics and Electrostatic Interactions Optimization
The rational improvement of the enzyme catalytic activity is one of the most significant
challenges in biotechnology. Most conventional strategies used to engineer enzymes …
challenges in biotechnology. Most conventional strategies used to engineer enzymes …
A coarse-grained xDLVO model for colloidal protein–protein interactions
Colloidal protein–protein interactions (PPIs) of attractive and repulsive nature modulate the
solubility of proteins, their aggregation, precipitation and crystallization. Such interactions …
solubility of proteins, their aggregation, precipitation and crystallization. Such interactions …
A dynamic hydrophobic core and surface salt bridges thermostabilize a designed three-helix bundle
C Nguyen, JT Young, GG Slade, RJ Oliveira… - Biophysical …, 2019 - cell.com
Thermostable proteins are advantageous in industrial applications, as pharmaceuticals or
biosensors, and as templates for directed evolution. As protein-design methodologies …
biosensors, and as templates for directed evolution. As protein-design methodologies …
Rational design of chymotrypsin inhibitor 2 by optimizing non-native interactions
F B. da Silva, V M. de Oliveira… - Journal of chemical …, 2019 - ACS Publications
Rational design of proteins via mutagenesis is crucial for several biotechnological
applications. A significant challenge of the computational strategies used to predict …
applications. A significant challenge of the computational strategies used to predict …
Electrostatic interaction optimization improves catalytic rates and thermotolerance on xylanases
Understanding the aspects that contribute to improving proteins' biochemical properties is of
high relevance for protein engineering. Properties such as the catalytic rate, thermal stability …
high relevance for protein engineering. Properties such as the catalytic rate, thermal stability …
The role of electrostatics and folding kinetics on the thermostability of homologous cold shock proteins
Understanding which aspects contribute to the thermostability of proteins is a challenge that
has persisted for decades, and it is of great relevance for protein engineering. Several types …
has persisted for decades, and it is of great relevance for protein engineering. Several types …
Directed-evolution mutations of adenine base editor ABE8e improve its DNA-binding affinity and protein stability
H Zhu, X Jiang, L Wang, Q Qin, M Song, Q Huang - bioRxiv, 2023 - biorxiv.org
Adenine base editors (ABEs), consisting of CRISPR-associated (Cas) nickase and
deaminase, can convert the A: T base pair to G: C. Previous studies have shown that ABE8e …
deaminase, can convert the A: T base pair to G: C. Previous studies have shown that ABE8e …
pH and charged mutations modulate cold shock protein folding and stability: A constant pH monte carlo study
The folding and stability of proteins is a fundamental problem in several research fields. In
the present paper, we have used different computational approaches to study the effects …
the present paper, we have used different computational approaches to study the effects …
An exploration of the SARS-CoV-2 spike receptor binding domain (RBD)–a complex palette of evolutionary and structural features
Abstract SARS-CoV-2 spike protein (S) is associated with the entry of virus inside the host
cell by recruiting its loop dominant receptor binding domain (RBD) and interacting with the …
cell by recruiting its loop dominant receptor binding domain (RBD) and interacting with the …
Molecular insights into TP53 mutation (p. Arg267Trp) and its connection to Choroid Plexus Carcinomas and Li-Fraumeni Syndrome
Z Abduljaleel - Genes & Genomics, 2024 - Springer
Abstract Background Choroid plexus carcinomas (CPCs) are rare malignant tumors
primarily affecting pediatric patients and often co-occur with Li-Fraumeni Syndrome (LFS) …
primarily affecting pediatric patients and often co-occur with Li-Fraumeni Syndrome (LFS) …