Transcript termination is essential for accurate gene expression and the removal of RNA
polymerase (RNAP) at the ends of transcription units. In bacteria, two mechanisms are …

The process of transcription termination is essential to proper expression of bacterial genes
and, in many cases, to the regulation of bacterial gene expression. Two types of bacterial …

We report here a system with which a correctly folded complete protein and its encoding
mRNA both remain attached to the ribosome and can be enriched for the ligand-binding …

Variants of λ repressor and cytochrome b562 translated from messenger RNAs without stop
codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and …

Here we applied ribosome display to in vitro selection and evolution of single-chain antibody
fragments (scFvs) from a large synthetic library (Human Combinatorial Antibody Library; …

Intrinsic termination of transcription in Escherichia coli involves the formation of an RNA
hairpin in the nascent RNA. This hairpin plays a central role in the release of the transcript …

The reliable forward engineering of genetic systems remains limited by the ad hoc reuse of
many types of basic genetic elements. Although a few intrinsic prokaryotic transcription …

A new algorithm called RNAMotif containing RNA structure and sequence constraints and a
thermodynamic scoring system was used to search for intrinsic rho-independent terminators …

Ternary complexes of DNA-dependent RNA polymerase with its DNA template and nascent
transcript are central intermediates in transcription. In recent years, several unusual …

Major bacterial small RNAs (sRNAs) regulate the translation and stability of target mRNAs
through base pairing with the help of the RNA chaperone Hfq. The Hfq-dependent sRNAs …