Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture
techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority …

In the last decades three-dimensional (3D) in vitro cancer models have been proposed as a
bridge between bidimensional (2D) cell cultures and in vivo animal models, the gold …

Three-dimensional (3D) tumor culture techniques are gaining popularity as in vitro models of
tumoral tissue analogues. Despite the widespread interest, need, and present-day effort …

To improve pathogenetic studies in cancer development and reliable preclinical testing of
anti-cancer treatments, three-dimensional (3D) cultures, including spheroids, have been …

Three-dimensional (3D) culture systems like tumor spheroids represent useful in vitro
models for drug screening and more broadly for cancer biology research, but the generation …

Organoids can recapitulate human-specific phenotypes and functions in vivo and have great
potential for research in development, disease modeling, and drug screening. Due to the …

Decades of research have shown that 2D cell culture studies are insufficient for preclinical
cancer diagnosis and treatment, and that cancer cells in 3D culture systems have better cell …

The wide use of 3D-organotypic cell models is imperative for advancing our understanding
of basic cell biological mechanisms. For this purpose, easy-to-use enabling technology is …

One of the most surprising differences observed when comparing cell cultures in 2D and 3D
is morphological dissimilarity and their evolution over time. Cells grown in a monolayer tend …

Preclinical research to predict the effects of drugs and chemicals on humans is commonly
carried out either by cell culture studies in vitro condition or on animals in vivo condition …