Long-term preservation of germ cells and gonadal tissues at ambient temperatures

P Comizzoli, X He, PC Lee - Reproduction and Fertility, 2022 - raf.bioscientifica.com
Objective To present an overview of different approaches and recent advances for long-term
preservation of germ cells and gonadal tissues at ambient temperatures. Methods Review of …

Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

P Comizzoli, O Amelkina, PC Lee - Molecular Reproduction and …, 2022 - Wiley Online Library
Long‐term preservation of sperm, oocytes, and gonadal tissues at ambient temperatures
has the potential to lower the costs and simplify biobanking in human reproductive medicine …

Alternative opportunities to collect semen and sperm cells for ex situ in vitro gene conservation in sheep

MA Mujitaba, I Egerszegi, G Kútvölgyi, S Nagy, N Vass… - Agriculture, 2022 - mdpi.com
The semen of domestic mammals is conventionally collected with an artificial vagina (AV) for
artificial insemination (AI) or for short-or long-term storage. However, the procedure has …

Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures

PC Lee, J Zahmel, K Jewgenow… - Journal of Assisted …, 2021 - Springer
Purpose To evaluate the DNA integrity and developmental potential of microwave-
dehydrated cat spermatozoa after storage at− 20° C for different time periods and/or …

Dried storage of sperm at supra-zero temperatures: An alternative for flow cytometric analysis under field conditions

J Rosero, AP dos Santos Silva, SCA Dos Santos… - Cryobiology, 2024 - Elsevier
In biotechnological processes such as chromosomal manipulation studies, semen has
become a reference in the ploidy verification of the evaluated material. However, the use of …

Long term DNA storage evaluation and methodology

R Lehto - 2021 - knowledgecommons.lakeheadu.ca
DNA preservation over the long term is a considerable problem for many fields that still lack
a viable, cost-effective, long-term solution. The considerable amount of genetic material that …