Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications

M Tan, C Liao, L Liang, X Yi, Z Zhou… - Frontiers in cellular and …, 2022 - frontiersin.org
After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people's lives. In
addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid …

Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review

OO Adewusi, CL Waldner… - Journal of …, 2024 - journals.sagepub.com
Rapid laboratory tests are urgently required to inform antimicrobial use in food animals. Our
objective was to synthesize knowledge on the direct application of long-read metagenomic …

Simultaneous detection of five foodborne pathogens using a mini automatic nucleic acid extractor combined with recombinase polymerase amplification and lateral …

B Jin, B Ma, J Li, Y Hong, M Zhang - Microorganisms, 2022 - mdpi.com
In recent years, foodborne disease outbreaks have caused huge losses to the economy and
have had severe impacts on public health. The accuracy and variety of detection techniques …

Rapid detection of Pseudomonas aeruginosa by recombinase polymerase amplification combined with CRISPR-Cas12a biosensing system

S Liu, S Huang, F Li, Y Sun, J Fu, F Xiao… - Frontiers in Cellular …, 2023 - frontiersin.org
Pseudomonas aeruginosa (P. aeruginosa) is an important bacterial pathogen involved in a
wide range of infections and antimicrobial resistance. Rapid and reliable diagnostic …

Fast, Simple, and Highly Specific Molecular Detection of Porphyromonas gingivalis Using Isothermal Amplification and Lateral Flow Strip Methods

D Ge, F Wang, Y Hu, B Wang, X Gao… - Frontiers in Cellular and …, 2022 - frontiersin.org
Porphyromonas gingivalis is an important oral pathogen that causes periodontal disease
and is difficult to culture under conventional conditions. Therefore, a reliable technique for …

Accurate, sensitive, and rapid detection of pseudomonas aeruginosa based on CRISPR/cas12b with one fluid-handling step

X Qiu, X Liu, R Wang, X Ma, L Han, J Yao… - Microbiology …, 2023 - Am Soc Microbiol
Pseudomonas aeruginosa is a major bacterial pathogen causing nosocomial infections and
accounts for morbidity and mortality among patients with cystic fibrosis. An accurate …

Development and Application of Rapid Clinical Visualization Molecular Diagnostic Technology for Cryptococcus neoformans/C. gattii Based on Recombinase …

L Wang, Y Wang, F Wang, M Zhao, X Gao… - Frontiers in cellular …, 2022 - frontiersin.org
Cryptococcus neoformans (C. neoformans)/C. gattii can easily invade the human central
nervous system and cause cryptococcal meningitis (CM). The clinical fatality rate of these …

Establishment of portable Pseudomonas aeruginosa detection platform based on one-tube CRISPR/Cas12a combined with recombinase polymerase amplification …

H Yang, A Liu, F Ma, X Gao, K Wang, Y Wang - Clinica Chimica Acta, 2024 - Elsevier
Pseudomonas aeruginosa, a common Gram-negative bacterium, is associated with diverse
diseases. Its increasing resistance to antibiotics presents challenges in clinical treatment …

A method for Porphyromonas gingivalis based on recombinase polymerase amplification and lateral flow strip technology

R Jiang, Y Zhang, X Ren, R Zhang, R Cheng… - Analytical Biochemistry, 2024 - Elsevier
Objective A practical visual detection method was established to detect Porphyromonas
gingivalis (P. gingivalis) by employing a combination of recombinase polymerase …

Establishment and application of a rapid visual diagnostic method for Streptococcus agalactiae based on recombinase polymerase amplification and lateral flow …

T Ji, Y Cai, Y Gao, G Wang, Y Miao, X Gao - Scientific Reports, 2024 - nature.com
This study aims to establish a rapid diagnostic method for Streptococcus agalactiae (GBS)
based on recombinase polymerase amplification (RPA) and lateral flow strips (LFS). The …