Challenges and opportunities in cryo-EM single-particle analysis
D Lyumkis - Journal of Biological Chemistry, 2019 - ASBMB
Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …
macromolecular objects and their assemblies. Although the techniques have been …
Cryo-EM in drug discovery: achievements, limitations and prospects
JP Renaud, A Chari, C Ciferri, W Liu… - Nature reviews Drug …, 2018 - nature.com
Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …
technique that can be used to determine the structure of biological macromolecules and …
New tools for automated cryo-EM single-particle analysis in RELION-4.0
D Kimanius, L Dong, G Sharov, T Nakane… - Biochemical …, 2021 - portlandpress.com
We describe new tools for the processing of electron cryo-microscopy (cryo-EM) images in
the fourth major release of the RELION software. In particular, we introduce VDAM, a …
the fourth major release of the RELION software. In particular, we introduce VDAM, a …
Structural and functional ramifications of antigenic drift in recent SARS-CoV-2 variants
Neutralizing antibodies (nAbs) elicited against the receptor binding site (RBS) of the spike
protein of wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are …
protein of wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are …
SARS-CoV-2 infection depends on cellular heparan sulfate and ACE2
TM Clausen, DR Sandoval, CB Spliid, J Pihl… - Cell, 2020 - cell.com
We show that SARS-CoV-2 spike protein interacts with both cellular heparan sulfate and
angiotensin-converting enzyme 2 (ACE2) through its receptor-binding domain (RBD) …
angiotensin-converting enzyme 2 (ACE2) through its receptor-binding domain (RBD) …
Long-primed germinal centres with enduring affinity maturation and clonal migration
Germinal centres are the engines of antibody evolution. Here, using human
immunodeficiency virus (HIV) Env protein immunogen priming in rhesus monkeys followed …
immunodeficiency virus (HIV) Env protein immunogen priming in rhesus monkeys followed …
Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability
PJM Brouwer, TG Caniels, K van der Straten… - Science, 2020 - science.org
The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has
had a large impact on global health, travel, and economy. Therefore, preventative and …
had a large impact on global health, travel, and economy. Therefore, preventative and …
Structural analysis of full-length SARS-CoV-2 spike protein from an advanced vaccine candidate
Vaccine efforts to combat the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-
2), which is responsible for the current coronavirus disease 2019 (COVID-19) pandemic, are …
2), which is responsible for the current coronavirus disease 2019 (COVID-19) pandemic, are …
Quadrivalent influenza nanoparticle vaccines induce broad protection
S Boyoglu-Barnum, D Ellis, RA Gillespie… - Nature, 2021 - nature.com
Influenza vaccines that confer broad and durable protection against diverse viral strains
would have a major effect on global health, as they would lessen the need for annual …
would have a major effect on global health, as they would lessen the need for annual …
[HTML][HTML] Antibodies from primary humoral responses modulate the recruitment of naive B cells during secondary responses
Vaccines generate high-affinity antibodies by recruiting antigen-specific B cells to germinal
centers (GCs), but the mechanisms governing the recruitment to GCs on secondary …
centers (GCs), but the mechanisms governing the recruitment to GCs on secondary …