Electron tomography of cells
The electron microscope has contributed deep insights into biological structure since its
invention nearly 80 years ago. Advances in instrumentation and methodology in recent …
invention nearly 80 years ago. Advances in instrumentation and methodology in recent …
[HTML][HTML] Big data in cryoEM: automated collection, processing and accessibility of EM data
Highlights•Cryogenic electron microscopy has been evolving towards a big data field.•Movie-
mode collection and automation has increased throughput.•Improved processing pipelines …
mode collection and automation has increased throughput.•Improved processing pipelines …
Structural diversity of bacterial flagellar motors
S Chen, M Beeby, GE Murphy, JR Leadbetter… - The EMBO …, 2011 - embopress.org
The bacterial flagellum is one of nature's most amazing and well‐studied nanomachines. Its
cell‐wall‐anchored motor uses chemical energy to rotate a microns‐long filament and …
cell‐wall‐anchored motor uses chemical energy to rotate a microns‐long filament and …
Architecture and assembly of the G ram‐positive cell wall
The bacterial cell wall is a mesh polymer of peptidoglycan–linear glycan strands cross‐
linked by flexible peptides–that determines cell shape and provides physical protection …
linked by flexible peptides–that determines cell shape and provides physical protection …
An inventory of the bacterial macromolecular components and their spatial organization
A Vendeville, D Larivière… - FEMS microbiology …, 2011 - academic.oup.com
Formerly regarded as small 'bags' of nucleic acids with randomly diffusing enzymes, bacteria
are organized by a sophisticated and tightly regulated molecular machinery. Here, we …
are organized by a sophisticated and tightly regulated molecular machinery. Here, we …
MreB: pilot or passenger of cell wall synthesis?
CL White, JW Gober - Trends in microbiology, 2012 - cell.com
The discovery that the bacterial cell shape determinant MreB is related to actin spurred new
insights into bacterial morphogenesis and development. The trafficking and mechanical …
insights into bacterial morphogenesis and development. The trafficking and mechanical …
Visualization of ATP synthase dimers in mitochondria by electron cryo-tomography
Electron cryo-tomography is a powerful tool in structural biology, capable of visualizing the
three-dimensional structure of biological samples, such as cells, organelles, membrane …
three-dimensional structure of biological samples, such as cells, organelles, membrane …
Using Tomoauto: a protocol for high-throughput automated cryo-electron tomography
Cryo-electron tomography (Cryo-ET) is a powerful three-dimensional (3-D) imaging
technique for visualizing macromolecular complexes in their native context at a molecular …
technique for visualizing macromolecular complexes in their native context at a molecular …
Peptidoglycan structure, biosynthesis, and dynamics during bacterial growth
A Walter, C Mayer - Extracellular sugar-based biopolymers matrices, 2019 - Springer
The peptidoglycan is the key structural component of the bacterial cell wall that rests outside
the cytoplasmic membrane and provides bacterial cells with physical strength and shape. It …
the cytoplasmic membrane and provides bacterial cells with physical strength and shape. It …
In situ imaging of bacterial secretion systems by electron cryotomography
The unique property of electron cryotomography (ECT) is its capability to resolve the
structure of macromolecular machines in their cellular context. The integration of ECT data …
structure of macromolecular machines in their cellular context. The integration of ECT data …