Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
[HTML][HTML] FLIM as a promising tool for cancer diagnosis and treatment monitoring
Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past
30 years and widely applied in biomedical engineering. Recent progress in fluorophore …
30 years and widely applied in biomedical engineering. Recent progress in fluorophore …
Classification of T-cell activation via autofluorescence lifetime imaging
AJ Walsh, KP Mueller, K Tweed, I Jones… - Nature biomedical …, 2021 - nature.com
The function of a T cell depends on its subtype and activation state. Here, we show that
imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be …
imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be …
[HTML][HTML] Metabolic heterogeneity in patient tumor-derived organoids by primary site and drug treatment
JT Sharick, CM Walsh, CM Sprackling, CA Pasch… - Frontiers in …, 2020 - frontiersin.org
New tools are needed to match cancer patients with effective treatments. Patient-derived
organoids offer a high-throughput platform to personalize treatments and discover novel …
organoids offer a high-throughput platform to personalize treatments and discover novel …
Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding
Organoid models have been used to address important questions in developmental and
cancer biology, tissue repair, advanced modelling of disease and therapies, among other …
cancer biology, tissue repair, advanced modelling of disease and therapies, among other …
Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array
Significance Fluorescence lifetime imaging microscopy (FLIM) of the metabolic co-enzyme
nicotinamide adenine dinucleotide (phosphate)[NAD (P) H] is a popular method to monitor …
nicotinamide adenine dinucleotide (phosphate)[NAD (P) H] is a popular method to monitor …
[HTML][HTML] Technologies to assess drug response and heterogeneity in patient-derived cancer organoids
Patient-derived cancer organoids (PDCOs) are organotypic 3D cultures grown from patient
tumor samples. PDCOs provide an exciting opportunity to study drug response and …
tumor samples. PDCOs provide an exciting opportunity to study drug response and …
Mapping spatiotemporal heterogeneity in tumor profiles by integrating high-throughput imaging and omics analysis
PA Patkulkar, AR Subbalakshmi, MK Jolly… - ACS …, 2023 - ACS Publications
Intratumoral heterogeneity associates with more aggressive disease progression and worse
patient outcomes. Understanding the reasons enabling the emergence of such …
patient outcomes. Understanding the reasons enabling the emergence of such …
Microphysiological head and neck cancer model identifies novel role of lymphatically secreted monocyte migration inhibitory factor in cancer cell migration and …
Regional metastasis of head and neck cancer (HNC) is prevalent (approximately 50% of
patients at diagnosis), yet the underlying drivers and mechanisms of lymphatic spread …
patients at diagnosis), yet the underlying drivers and mechanisms of lymphatic spread …
[HTML][HTML] Label-free metabolic and structural profiling of dynamic biological samples using multimodal optical microscopy with sensorless adaptive optics
Label-free optical microscopy has matured as a noninvasive tool for biological imaging; yet,
it is criticized for its lack of specificity, slow acquisition and processing times, and weak and …
it is criticized for its lack of specificity, slow acquisition and processing times, and weak and …