Manipulation of hormone biosynthetic genes in transgenic plants

P Hedden, AL Phillips - Current Opinion in Biotechnology, 2000 - Elsevier
Modification of plant hormone biosynthesis through the introduction of bacterial genes is a
natural form of genetic engineering, which has been exploited in numerous studies on …

RNA silencing: no mercy for viruses?

CH Lecellier, O Voinnet - Immunological Reviews, 2004 - Wiley Online Library
'RNA silencing'is a highly conserved mechanism leading to suppression of gene expression
through nucleotide sequence‐specific interactions that are mediated by 21–24 nucleotide …

Distinct features of post‐transcriptional gene silencing by antisense transgenes in single copy and inverted T‐DNA repeat loci

M Stam, R De Bruin, R Van Blokland… - The Plant …, 2000 - Wiley Online Library
The application of antisense transgenes in plants is a powerful tool to inhibit gene
expression. The underlying mechanism of this inhibition is still poorly understood. High …

Neither inverted repeat T‐DNA configurations nor arrangements of tandemly repeated transgenes are sufficient to trigger transgene silencing

B Lechtenberg, D Schubert, A Forsbach… - The Plant …, 2003 - Wiley Online Library
Transgene expression was analysed in Arabidopsis T‐DNA transformants carrying defined
numbers and arrangement of different reporter genes. All transgenes were placed under the …

Homology-dependent gene silencing mechanisms in fungi

C Cogoni - Annual Reviews in Microbiology, 2001 - annualreviews.org
▪ Abstract Homology-dependent gene silencing (HDGS) is a ubiquitous phenomenon
among fungi, plants, and animals. Gene silencing can be triggered and can affect artificially …

RNA degradation and models for post-transcriptional gene silencing

F Meins - Plant Gene Silencing, 2000 - Springer
Post-transcriptional gene silencing (PTGS) is a form of stable but potentially reversible
epigenetic modification, which frequently occurs in transgenic plants. The interaction in trans …

Transgenic expression of a Δ12-epoxygenase gene in Arabidopsis seeds inhibits accumulation of linoleic acid

S Singh, S Thomaeus, M Lee, S Stymne, A Green - Planta, 2001 - Springer
The Crepis palaestina cDNA Cpal2 encodes a Δ12-epoxygenase that can catalyse the
synthesis of 12, 13-epoxy-cis-9-octadecenoic acid (18: 1E) from linoleic acid (18: 2). When …

[HTML][HTML] Dissecting the mechanisms of posttranscriptional gene silencing: divide and conquer

SR Grant - Cell, 1999 - cell.com
It is now routine to construct trangenes with defined promoters and terminators and express
them in a variety of organisms. However, some introduced transgenes are not expressed as …

RNA silencing

SW Ding - Current Opinion in Biotechnology, 2000 - Elsevier
Gene silencing through the increased degradation of mRNA appears to represent a novel
cellular pathway that is functional in a broad range of organisms. Recent work has …

High frequency induction of RNA-mediated resistance against Cucumber mosaic virus using inverted repeat constructs

YK Chen, D Lohuis, R Goldbach, M Prins - Molecular Breeding, 2004 - Springer
The application of RNA-mediated resistance against Cucumber mosaic virus (CMV) by
using single transgene constructs generally results in only a small portion of resistant …