A transgenic perspective on plant functional genomics
A Pereira - Transgenic research, 2000 - Springer
Transgenic crops are very much in the news due to the increasing public debate on their
acceptance. In the scientific community though, transgenic plants are proving to be powerful …
acceptance. In the scientific community though, transgenic plants are proving to be powerful …
Sense-and antisense-mediated gene silencing in tobacco is inhibited by the same viral suppressors and is associated with accumulation of small RNAs
F Di Serio, H Schöb, A Iglesias… - Proceedings of the …, 2001 - National Acad Sciences
Antisense-mediated gene silencing (ASGS) and posttranscriptional gene silencing (PTGS)
with sense transgenes markedly reduce the steady-state mRNA levels of endogenous …
with sense transgenes markedly reduce the steady-state mRNA levels of endogenous …
Inverted repeat of a heterologous 3′‐untranslated region for high‐efficiency, high‐throughput gene silencing
DA Brummell, PJ Balint‐Kurti, MH Harpster… - The Plant …, 2003 - Wiley Online Library
This report describes a method for the easy generation of inverted repeat constructs for the
silencing of genes of unknown sequence which is applicable to high‐throughput studies …
silencing of genes of unknown sequence which is applicable to high‐throughput studies …
RNA target sequences promote spreading of RNA silencing
H Van Houdt, A Bleys, A Depicker - Plant Physiology, 2003 - academic.oup.com
It is generally recognized that a silencing-inducing locus can efficiently reduce the
expression of genes that give rise to transcripts partially homologous to those produced by …
expression of genes that give rise to transcripts partially homologous to those produced by …
Dynamics of the VIGS-Mediated Chimeric Silencing of the Nicotiana benthamiana ChlH Gene and of the Tobacco Mosaic Virus Vector
JB Hiriart, EM Aro, K Lehto - Molecular plant-microbe …, 2003 - Am Phytopath Society
The ChlH gene, encoding for the H subunit of the magnesium chelatase enzyme, was
silenced in Nicotiana bentahamiana plants by virus-induced gene silencing (VIGS), using …
silenced in Nicotiana bentahamiana plants by virus-induced gene silencing (VIGS), using …
Reconstruction of Arabidopsis thaliana fully integrated small RNA pathway
SA Jamalkandi, A Masoudi-Nejad - Functional & integrative genomics, 2009 - Springer
Small non-protein-coding RNAs (ncRNAs) have acquired diverse functions in plant and
animals development. These riboregulators have been confirmed to cooperate in plant …
animals development. These riboregulators have been confirmed to cooperate in plant …
Potato virus X amplicon‐mediated silencing of nuclear genes
SM Angell, DC Baulcombe - The Plant Journal, 1999 - Wiley Online Library
Transgenic expression of a replicating potato virus X (PVX) construct (termed an 'amplicon')
reproducibly and consistently activates post‐transcriptional gene silencing (PTGS) in every …
reproducibly and consistently activates post‐transcriptional gene silencing (PTGS) in every …
Methods of double-stranded RNA-mediated gene inactivation in Arabidopsis and their use to define an essential gene in methionine biosynthesis
JZ Levin, AJ de Framond, A Tuttle, MW Bauer… - Plant Molecular …, 2000 - Springer
Controlled down-regulation of endogenous plant gene expression is a useful tool, but
antisense and sense silencing lack predictability. Recent studies show that expression of …
antisense and sense silencing lack predictability. Recent studies show that expression of …
Transgene silencing of invertedly repeated transgenes is released upon deletion of one of the transgenes involved
SD Buck, MV Montagu, A Depicker - Plant Molecular Biology, 2001 - Springer
To analyse experimentally the correlation between transgene silencing and the presence of
an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the β …
an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the β …