An Intron Sense Suppression Construct Targeting Soybean FAD2-1 Requires a Double-Stranded RNA-Producing Inverted Repeat T-DNA Insert

A Mroczka, PD Roberts, JAJ Fillatti, BE Wiggins… - Plant …, 2010 - academic.oup.com
We demonstrate that the transformation of soybean (Glycine max) with sense suppression
constructs using intron sequences from the fatty acid oleyl Δ12 desaturase gene FAD2-1A …

Stochastic and nonstochastic post-transcriptional silencing of chitinase and β-1, 3-glucanase genes involves increased RNA turnover—possible role for ribosome …

H Holtorf, HSC Kunz, R Waldvogel, F Meins Jr - The Plant Cell, 1999 - academic.oup.com
Stochastic and nonstochastic post-transcriptional gene silencing (PTGS) in Nicotiana
sylvestris plants carrying tobacco class I chitinase (CHN) and β-1, 3-glucanase transgenes …

RNA 沉默机制及其抗病毒应用

牛颜冰, 青玲, 周雪平 - 中国生物工程杂志, 2004 - manu60.magtech.com.cn
RNA 沉默是发生在植物(转录后基因沉默或共抑制), 动物(RNA 干扰) 和真菌(消除作用)
等真核生物细胞中的一种对外源遗传因子(转座子, 转基因或病毒) 的特异性和高效率的降解机制 …

Introns reduce transitivity proportionally to their length, suggesting that silencing spreads along the pre‐mRNA

L Vermeersch, N De Winne, A Depicker - The Plant Journal, 2010 - Wiley Online Library
Endogenes rarely support transitive silencing, whereas most transgenes generally allow the
spread of silencing to occur along the primary target. To determine whether the presence of …

Degreening behavior in 'Fallglo'and 'Lee× Orlando'is correlated with differential expression of ethylene signaling and biosynthesis genes

KJ John-Karuppiah, JK Burns - Postharvest biology and technology, 2010 - Elsevier
Two citrus types ('Fallglo'and 'Lee× Orlando') exhibiting differential fruit degreening
response when treated with ethylene were selected. Fruit were harvested at commercial …

Intron hairpin and transitive RNAi mediated silencing of orfH522 transcripts restores male fertility in transgenic male sterile tobacco plants expressing orfH522

NR Nizampatnam, V Dinesh Kumar - Plant molecular biology, 2011 - Springer
The present work was aimed at developing vector construct (s) suitable for restoring fertility
in transgenic male sterile tobacco plants expressing male-sterility-inducing ORFH522 in …

[PDF][PDF] Regulation of metabolite synthesis in plants

LJ Cseke, PB Kaufman, A Kirakosyan… - Natural products from …, 2006 - academia.edu
In Chapter 2, we pointed out the importance of knowing the sequence of substrates and the
respective enzymes involved in the biochemical pathways that lead to the synthesis of given …

马铃薯Y 病毒CP 基因5′ 端和3′ 端反向重复结构介导的抗病性研究

李鹏, 宋云枝, 刘晓玲, 朱常香, 温孚江 - 植物病理学报, 2007 - cqvip.com
本研究克隆了来源于马铃薯Y 病毒坏死株系(PVY^ N) 外壳蛋白(CP) 基因(PVY^ N, CP)
5'端和3'端的反向重复cDNA 序列, 并构建了植物表达载体pROKII-5'IR 和pROKII-3'IR …

Synthetic genes and genetic constructs

MW Graham, RN Rice - US Patent 8,048,670, 2011 - Google Patents
The present invention relates generally to synthetic genes for modifying endogenous gene
expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic …

[HTML][HTML] The mechanism of graft transmission of sense and antisense gene silencing in tomato plants

NA Shaharuddin, Y Han, H Li, D Grierson - FEBS letters, 2006 - Elsevier
We investigated the effect of target mRNA level on grafting-transmitted gene silencing in
tomato plants by using a strong ACC oxidase 1 (ACO1) silencer as the stock and transgenic …