Industrial chemical production largely relies on fossil fuels, resulting in the unavoidable
release of carbon dioxide (CO2) into the atmosphere. The concept of a circular carbon …

Microbial response to toxic fluoride anion has traditionally been studied by adding inorganic
fluoride salts to growth media. Fluoride is known to spontaneously transit the membrane as …

A synthetic biology toolkit, exploiting clustered regularly interspaced short palindromic
repeats (CRISPR) and modified CRISPR-associated protein (Cas) base-editors, was …

Thymidylate kinases (TMPKs) play an essential role in DNA biosynthesis across all domains
of life by catalyzing dTMP phosphorylation to dTDP. In Pseudomonas putida KT2440, a …

Background Interest in the use of engineered microbes to deliver therapeutic activities has
increased in recent years. The probiotic yeast Saccharomyces boulardii has been …

Paenibacillus polymyxa is a non‐pathogenic, Gram‐positive bacterium endowed with a rich
and versatile metabolism. However interesting, this bacterium has been seldom used for …

Understanding diverse bacterial nutritional requirements and responses is foundational in
microbial research and biotechnology. In this study, we employed knowledge-enriched …

Pseudomonas putida, a microbial host widely adopted for metabolic engineering, processes
glucose through convergent peripheral pathways that ultimately yield 6-phosphogluconate …

To streamline the analysis and visualization of bacterial growth and gene expression data
obtained by microtitre plate readers, we developed BactEXTRACT, an intuitive, easy-to-use …

Background Recording and analyzing microbial growth is a routine task in the life sciences.
Microplate readers that record dozens to hundreds of growth curves simultaneously are …