It has been estimated that 650,000 protein–protein interactions exist in the human
interactome (Stumpf et al., 2008 [1]), a subset of all possible macromolecular partnerships …

Many proteins can be split into fragments that spontaneously reassemble, without covalent
linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment …

Splitting bioactive proteins into conditionally reconstituting fragments is a powerful strategy
for building tools to study and control biological systems. However, split proteins often …

Directed evolution is a robust and powerful tool for engineering new and/or improved
functions in biomolecules for therapeutic and industrial applications, as well as to uncover …

Highlights•Established applications of fluorescent proteins are fundamental to modern
research.•A number fluorescent protein-based biosensing technologies have emerged in …

Protein engineering is widely used to generate proteins with novel or enhanced function.
However, manipulating protein function in the laboratory can prove laborious, protracted and …

The dynamics of synaptic vesicles (SVs) within presynaptic domains are tightly controlled by
synapsin1 phosphorylation; however, the mechanism underlying the anchoring of synapsin1 …

Protein stability affects the physiological functions of proteins and is also a desirable trait in
many protein engineering tasks, yet improving protein stability is challenging because of …

Most proteins are only barely stable, which impedes research, complicates therapeutic
applications, and makes proteins susceptible to pathologically destabilizing mutations. Our …

Protein stability is not only fundamental for experimental, industrial, and therapeutic
applications, but is also the baseline for evolving novel protein functions. For decades …