A fresh start for IVM: capacitating the oocyte for development using pre-IVM

RB Gilchrist, TM Ho, M De Vos… - Human …, 2024 - academic.oup.com
BACKGROUND While oocyte IVM is practiced sporadically it has not achieved widespread
clinical practice globally. However, recently there have been some seminal advances in our …

Embryo biotechnologies in sheep: Achievements and new improvements

L Falchi, S Ledda, MT Zedda - Reproduction in domestic …, 2022 - Wiley Online Library
To date, large‐scale use of multiple ovulation and embryo transfer (MOET) programmes in
ovine species is limited due to unpredictable results and high costs of hormonal stimulation …

One-step automated bioprinting-based method for cumulus-oocyte complex microencapsulation for 3D in vitro maturation

A Mastrorocco, L Cacopardo, NA Martino, D Fanelli… - PLoS …, 2020 - journals.plos.org
Three-dimensional in vitro maturation (3D IVM) is a promising approach to improve IVM
efficiency as it could prevent cumulus-oocyte complex (COC) flattening and preserve its …

Sequential IVM by CNP preincubation and cooperating of PGE2 with AREG enhances developmental competence of SCNT reconstructs in goat

N Assareh, M Shahemabadi, SR Varnosfaderani… - Scientific Reports, 2022 - nature.com
Developmental competence of in vitro matured cumulus oocyte complexes (COCs) in
conventional IVM (C. IVM) is lower than in vivo maturated COCs and is related to …

In vitro production of small ruminant embryos: Latest improvements and further research

JMG Souza-Fabjan, RITP Batista… - Reproduction …, 2021 - CSIRO Publishing
This review presents the latest advances in and main obstacles to the application of in vitro
embryo production (IVEP) systems in small ruminants. This biotechnology is an extremely …

IVM advances for early antral follicle-enclosed oocytes coupling reproductive tissue engineering to inductive influences of human chorionic gonadotropin and ovarian …

A Peserico, C Di Berardino, G Capacchietti… - International Journal of …, 2023 - mdpi.com
In vitro maturation (IVM) is not a routine assisted reproductive technology (ART) for oocytes
collected from early antral (EA) follicles, a large source of potentially available gametes …

Developmental competence of IVF and SCNT goat embryos is improved by inhibition of canonical WNT signaling

M Sadeghi, MR Andani, M Hajian, N Sanei… - Plos one, 2023 - journals.plos.org
The specific role of the canonical WNT/β-catenin signaling pathway during the
preimplantation development of goat remains unclear. Our objective was to investigate the …

Combination of CNP, MT and FLI during IVM significantly improved the quality and development abilities of bovine oocytes and IVF-derived embryos

P Zhang, B Yang, X Xu, H Zhang, X Feng, H Hao, W Du… - Antioxidants, 2023 - mdpi.com
Oocyte maturation is a critical step in the completion of female gametogenesis in the ovary;
thus, for subsequent fertilization and embryogenesis. Vitrification of embryo also has been …

Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI …

AR Bartková, L Němcová, V Kinterová… - Journal of Ovarian …, 2024 - Springer
Background Oocytes of large animal species isolated from small ovarian follicles (< 2 mm)
are less competent to support early embryonic development after in vitro maturation and …

PGE2 supplementation of oocyte culture media improves the developmental and cryotolerance performance of bovine blastocysts derived from a serum-free in vitro …

G Charpigny, B Marquant-Le Guienne… - Frontiers in Cell and …, 2021 - frontiersin.org
The culture media used throughout the in vitro production (IVP) of bovine embryos remain
complex. The serum added to culture media in order to improve embryo development …