Recent Advances in Chiral Selectors Immobilization and Chiral Mobile Phase Additives in Liquid Chromatographic Enantio-separations; A Review

AE Ibrahim, NA El Gohary, D Aboushady… - … of Chromatography A, 2023 - Elsevier
For decades now, the separation of chiral enantiomers of drugs has been gaining the
interest and attention of researchers. In 1991, the first guidelines for development of chiral …

Materials, workflows and applications of IMAC for phosphoproteome profiling in the recent decade: A review

R Tang, Q Bai, S Ma, J Ou - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
The last ten years have witnessed the increasingly notable advances in immobilized metal
ion chromatography (IMAC) for phosphoproteomic profiling. Protein phosphorylation is an …

Applications of cryostructures in the chromatographic separation of biomacromolecules

N Babanejad, K Mfoafo, E Zhang, Y Omidi… - … of Chromatography A, 2022 - Elsevier
Macroporous-interconnected cryostructures are prepared via cryoprocess approaches,
where polymerization, crosslinking-polymerization, or even chain aggregation is primarily …

Application of composite cryogels in downstream processing-A review

S Hajizadeh - Reactive and Functional Polymers, 2023 - Elsevier
The demand for efficient separation and purification methods has inspired the development
of new techniques and materials for bioseparation. Conventional chromatographic columns …

Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: insights from structure-function relationship for novel applications

K Kachhawaha, S Singh, K Joshi, P Nain… - Preparative …, 2023 - Taylor & Francis
The formation of inclusion bodies (IBs) during expression of recombinant therapeutic
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …

Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids

J Ribeiro, MÂ Luís, B Rodrigues, FM Santos… - Gels, 2024 - mdpi.com
The increasing demand for highly pure biopharmaceuticals has put significant pressure on
the biotechnological industry to innovate in production and purification processes. Nucleic …

Recent progress in analytical strategies of arsenic-binding proteomes in living systems

J Liu, B Chen, R Zhang, Y Li, R Chen, S Zhu… - Analytical and …, 2023 - Springer
Arsenic (As) is one of the most concerning elements due to its high exposure risks to
organisms and ecosystems. The interaction between arsenicals and proteins plays a pivotal …

Application of peptide biomarkers in life analysis based on liquid chromatography–mass spectrometry technology

T Han, H Cong, B Yu, Y Shen - Biofactors, 2022 - Wiley Online Library
Biomedicine is developing rapidly in the 21st century. Among them, the qualitative and
quantitative analysis of peptide biomarkers is of considerable importance for the diagnosis …

Macroporous epoxy-based monoliths functionalized with anti-CD63 nanobodies for effective isolation of extracellular vesicles in urine

J Neumair, C D'Ercole, M De March, M Elsner… - International journal of …, 2023 - mdpi.com
Extracellular vesicles (EVs) have enormous potential for the implementation of liquid biopsy
and as effective drug delivery means, but the fulfilment of these expectations requires …

Comparative analysis of trilobal capillary‐channeled polymer fiber columns with superficially porous and monolithic phases toward reversed‐phase protein …

LS Billotto, RK Marcus - Journal of Separation Science, 2022 - Wiley Online Library
A trilobal capillary‐channeled polymer fiber stationary phase is evaluated for its
performance for intact protein separations under reversed‐phase high‐performance liquid …