CRISPR interference (CRISPRi) is a powerful tool to link essential and nonessential genes
to specific phenotypes and to explore their functions. Here we describe a protocol for …

Deciphering gene function is fundamental to engineering of microbiology. The clustered
regularly interspaced short palindromic repeats (CRISPR) system has been adapted for …

The ability to switch a gene from off to on and monitor dynamic changes provides a powerful
approach for probing gene function and elucidating causal regulatory relationships. Here …

The optimization of cellular functions often requires the balancing of gene expression, but
the physical construction and screening of alternative designs are costly and time …

The CRISPR/Cas9 systems have been developed as tools for genetic engineering and
metabolic engineering in various organisms. In this review, various aspects of CRISPR/Cas9 …

For the fungal pathogen Candida albicans, genetic overexpression readily occurs via a
diversity of genomic alterations, such as aneuploidy and gain-of-function mutations, with …

Background CRISPR/Cas9-mediated transcriptional interference (CRISPRi) enables
programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene …

Increased human population and the rapid decline of fossil fuels resulted in a global
tendency to look for alternative fuel sources. Environmental concerns about fossil fuel …

The Yca1 metacaspase was discovered due to its role in the regulation of apoptosis in
Saccharomyces cerevisiae. However, the mechanisms that drive apoptosis in yeast remain …

ABSTRACT CRISPR interference (CRISPRi) is a powerful tool to study cellular physiology
under different growth conditions, and this technology provides a means for screening …