Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue

JA Aronowitz, RA Lockhart, CS Hakakian - Springerplus, 2015 - Springer
Clinical use of adipose-derived stem cells (ASCs) for a variety of indications is rapidly
expanding in medicine. Most commonly, ASCs are isolated at the point of care from …

[HTML][HTML] Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art

E Oberbauer, C Steffenhagen, C Wurzer, C Gabriel… - Cell Regeneration, 2015 - Elsevier
In the past decade, adipose tissue became a highly interesting source of adult stem cells for
plastic surgery and regenerative medicine. The isolated stromal vascular fraction (SVF) is a …

Adipose-derived mesenchymal stem cells from the elderly exhibit decreased migration and differentiation abilities with senescent properties

M Liu, H Lei, P Dong, X Fu, Z Yang, Y Yang… - Cell …, 2017 - journals.sagepub.com
Adipose-derived stem cells (ASCs) can be applied extensively in the clinic because they can
be easily isolated and cause less donor-site morbidity; however, their application can be …

Overview of current adipose-derived stem cell (ADSCs) processing involved in therapeutic advancements: flow chart and regulation updates before and after COVID …

L Mazini, M Ezzoubi, G Malka - Stem cell research & therapy, 2021 - Springer
Adipose-derived stem cells (ADSCs) have raised big interest in therapeutic applications in
regenerative medicine and appear to fulfill the criteria for a successful cell therapy. Their low …

Impact of the different preparation methods to obtain human adipose-derived stromal vascular fraction cells (AD-SVFs) and human adipose-derived mesenchymal …

P Gentile, C Calabrese, B De Angelis… - International journal of …, 2019 - mdpi.com
Autologous therapies using adipose-derived stromal vascular fraction (AD-SVFs) and adult
adipose-derived mesenchymal stem cells (AD-MSCs) warrant careful preparation of the …

Nanofat cell aggregates: a nearly constitutive stromal cell inoculum for regenerative site-specific therapies

B Sesé, JM Sanmartín, B Ortega… - Plastic and …, 2019 - journals.lww.com
Background: Recent technology developed by Tulip Medical Products allows clinicians to
mechanically disaggregate fat tissue into small fat particles known as nanofat. The present …

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

JA van Dongen, AJ Tuin, M Spiekman… - Journal of tissue …, 2018 - Wiley Online Library
Intraoperative application of the stromal vascular fraction (SVF) of adipose tissue requires a
fast and efficient isolation procedure of adipose tissue. This review was performed to …

Mesenchymal stem cell implantation in knee osteoarthritis: an assessment of the factors influencing clinical outcomes

YS Kim, YJ Choi, YG Koh - The American journal of sports …, 2015 - journals.sagepub.com
Background: Several clinical studies have reported on cell-based treatment using
mesenchymal stem cells (MSCs) for cartilage regeneration in knee osteoarthritis (OA) …

State of the field: cellular and exosomal therapeutic approaches in vascular regeneration

EP Tracy, V Stielberg, G Rowe… - American Journal …, 2022 - journals.physiology.org
Pathologies of the vasculature including the microvasculature are often complex in nature,
leading to loss of physiological homeostatic regulation of patency and adequate perfusion to …

Adipose tissue‐derived extracellular matrix hydrogels as a release platform for secreted paracrine factors

JA van Dongen, V Getova, LA Brouwer… - Journal of tissue …, 2019 - Wiley Online Library
Fat grafting is an established clinical intervention to promote tissue repair. The role of the
fat's extracellular matrix (ECM) in regeneration is largely neglected. We investigated in vitro …