Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI

VY Rawe, HU Boudri, CA Sedó, M Carro… - Reproductive …, 2010 - Elsevier
Magnetic activated cell sorting (MACS) with annexin V microbeads recognizes externalized
phosphatidylserine (PS) residues on the surface of apoptotic spermatozoa. The successful …

DNA damage of human spermatozoa in assisted reproduction: origins, diagnosis, impacts and safety

B Ozmen, N Koutlaki, M Youssry, K Diedrich… - Reproductive …, 2007 - Elsevier
Sperm DNA contributes half the offspring's genomic material and abnormal DNA can lead to
derangements in the reproductive process. Normal sperm genetic material is required for …

DNA fragmentation in human sperm after magnetic-activated cell sorting

S Bucar, A Gonçalves, E Rocha, A Barros… - Journal of assisted …, 2015 - Springer
Purpose As fertilization with unselected apoptotic spermatozoa may contribute to failures in
assisted reproductive techniques, it has become essential to remove this type of sperm in …

Sperm syringe: 3D sorting platform for assisted reproduction

F Yazdan Parast, MK O'Bryan… - Advanced Materials …, 2022 - Wiley Online Library
Selection of high‐quality sperm is crucial to assisted reproduction. However, conventional
clinical methods for sperm selection are manual and prone to operator errors. This article …

PICSI vs. MACS for abnormal sperm DNA fragmentation ICSI cases: a prospective randomized trial

E Hasanen, K Elqusi, S ElTanbouly… - Journal of assisted …, 2020 - Springer
Purpose To know which sperm selection technique, physiological intracytoplasmic sperm
injection (PICSI) or magnetic-activated cell sorting (MACS), is better for the selection of …

Teratozoospermia: Its association with sperm DNA defects, apoptotic alterations, and oxidative stress

O Ammar, M Mehdi, M Muratori - Andrology, 2020 - Wiley Online Library
This study was carried out to evaluate the level of nuclear sperm DNA damage in men with
isolated polymorphic teratozoospermia and examining its relationship with apoptosis and …

Use of testicular sperm for intracytoplasmic sperm injection in men with high sperm DNA fragmentation: a SWOT analysis

SC Esteves, M Roque, N Garrido - Asian journal of andrology, 2018 - journals.lww.com
Spermatozoa retrieved from the testis of men with high levels of sperm DNA fragmentation
(SDF) in the neat semen tend to have better DNA quality. Given the negative impact of SDF …

Markers of oxidative stress and sperm chromatin integrity

A Agarwal, AC Varghese, RK Sharma - Molecular endocrinology: Methods …, 2009 - Springer
Oxidative stress (OS) is an imbalance between the amount of reactive oxygen species
(ROS) produced and the ability of the antioxidants to scavenge these. OS has been …

Mitochondria-targeted compounds to assess and improve human sperm function

S Escada-Rebelo, MI Cristo… - Antioxidants & Redox …, 2022 - liebertpub.com
Significance: Currently 10%–15% of couples in reproductive age face infertility issues. More
importantly, male factor contributes to 50% of these cases (either alone or in combination …

Magnetic‐activated cell sorting before density gradient centrifugation improves recovery of high‐quality spermatozoa

TS Berteli, MG Da Broi, WP Martins, RA Ferriani… - …, 2017 - Wiley Online Library
Recent studies have evaluated the use of magnetic‐activated cell sorting (MACS) to reduce
apoptotic spermatozoa and improve sperm quality. However, the efficiency of using MACS …