Mutational analysis of the conserved domains of a T-region border repeat of Agrobacterium tumefaciens

MJJ Van Haaren, NJA Sedee, HA De Boer… - Plant molecular …, 1989 - Springer
Left-and right-border repeats, which surround the T-region, contain two conserved regions
separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded …

[PDF][PDF] Mechanistic insight into Type I restriction endonucleases

J Youell, K Firman - Front Biosci, 2012 - article.imrpress.com
Introduction 2.1. Genetic organization and nomenclature 2.2. Families of type I restriction
enzymes 2.3. DNA methylation and cleavage 3. DNA translocation and molecular motor …

Analysis of type I restriction modification systems in the Neisseriaceae: genetic organization and properties of the gene products

A Piekarowicz, A Kłyż, A Kwiatek… - Molecular …, 2001 - Wiley Online Library
The hsd locus (host specificity of DNA) was identified in the Neisseria gonorrhoeae genome.
The DNA fragment encoding this locus produced an active restriction and modification (R/M) …

Identification of a subdomain within DNA‐(cytosine‐C5)‐methyltransferases responsible for the recognition of the 5′ part of their DNA target.

C Lange, C Wild, TA Trautner - The EMBO Journal, 1996 - embopress.org
In previous work on DNA‐(cytosine‐C5)‐methyltransferases (C5‐MTases), domains had
been identified which are responsible for the sequence specificity of the different enzymes …

Promiscuous DNA cleavage by HpyAII endonuclease is modulated by the HNH catalytic residues

S Kumar, S Bangru, R Kumar, DN Rao - Bioscience Reports, 2020 - portlandpress.com
Helicobacter pylori is a carcinogenic bacterium that is responsible for 5.5% of all human
gastric cancers. H. pylori codes for an unusually large number of restriction–modification (R …

Characterization of mutations of the bacteriophage P1 mod gene encoding the recognition subunit of the EcoP1 restriction and modification system

DN Rao, H Eberle, TA Bickle - Journal of bacteriology, 1989 - Am Soc Microbiol
This study characterized several mutations of the bacteriophage P1 mod gene. This gene
codes for the subunit of the EcoP1 restriction enzyme that is responsible for DNA sequence …

A novel gene-fusing vector: construction of a 5′-GGmCC-specific chimeric methyltransferase, M· BspRI/M· BsuRI

SC Kim, G Pósfai, W Szybalski - Gene, 1991 - Elsevier
A vector was designed to allow predetermined and precise fusion between two cloned
genes by constructing a cassette with two unique class-IIS restriction sites, 5′—ACCTGC …

Construction of intra-domain chimeras of aminoacyl-tRNA synthetases

RM Starzyk, P Schimmel - Journal of Biomolecular Structure and …, 1989 - Taylor & Francis
To explore new approaches to enzyme engineering, intra-domain chimeras of two
aminoacyltRNA synthetases were constructed. Connections were made within the …

Four new type I restriction enzymes identified in Escherichia coli clinical isolates

JKA Kasarjian, Y Kodama, M Iida… - Nucleic Acids …, 2005 - academic.oup.com
Using a plasmid transformation method and the RM search computer program, four type I
restriction enzymes with new recognition sites and two isoschizomers (EcoBI and Eco377I) …

[图书][B] Characterization of a new restriction-modification system, the BcgI system of Bacillus coagulans

H Kong - 1998 - search.proquest.com
Abstract The BcgI and other BcgI-like restriction endonucleases differ from other types of
endonucleases in that they cleave double-stranded DNA on both sides of their recognition …