Computational analysis of human skeletal remains in ancient DNA and forensic genetics

A Childebayeva, EI Zavala - Iscience, 2023 - cell.com
Degraded DNA is used to answer questions in the fields of ancient DNA (aDNA) and
forensic genetics. While aDNA studies typically center around human evolution and past …

Review of direct PCR and Rapid DNA approaches to streamline sexual assault kit testing

M Harrel, AS Holmes - Journal of Forensic Sciences, 2022 - Wiley Online Library
Crime laboratories have been faced with large casework backlogs due to lengthy
processing times, limited resources and scientists, and rising crime rates. Evidence related …

High DNA yield from metatarsal and metacarpal bones from Slovenian Second World War skeletal remains

T Zupanc, E Podovšovnik, M Obal, IZ Pajnič - Forensic Science International …, 2021 - Elsevier
DNA yield varies by anatomical region, and the selection of bone types that yield maximum
recovery of DNA is important to maximize the success of human identification of skeletal …

Comparison of DNA preservation between adult and non-adult ancient skeletons

A Šuligoj, S Mesesnel, T Leskovar… - International Journal of …, 2022 - Springer
Studies evaluating DNA preservation in non-adults, or comparing preservation in adults and
non-adults, are very rare. This study compares the preservation of DNA in the skeletal …

[HTML][HTML] Detecting DNA damage in stored blood samples

K Schulze Johann, H Bauer, P Wiegand… - … Science, Medicine and …, 2023 - Springer
Several commercially available quantitative real-time PCR (qPCR) systems enable highly
sensitive detection of human DNA and provide a degradation index (DI) to assess DNA …

[HTML][HTML] Direct and secondary transfer of touch dna on a credit card: evidence evaluation given activity level propositions and application of bayesian networks

M Onofri, C Altomare, S Severini, F Tommolini… - Genes, 2023 - mdpi.com
In a judiciary setting, questions regarding the mechanisms of transfer, persistence, and
recovery of DNA are increasingly more common. The forensic expert is now asked to …

DNA and RNA profiling of excavated human remains with varying postmortem intervals

M van den Berge, D Wiskerke, RRR Gerretsen… - International journal of …, 2016 - Springer
When postmortem intervals (PMIs) increase such as with longer burial times, human
remains suffer increasingly from the taphonomic effects of decomposition processes such as …

Evaluation of four commercial quantitative real-time PCR kits with inhibited and degraded samples

AS Holmes, R Houston, K Elwick, D Gangitano… - International journal of …, 2018 - Springer
DNA quantification is a vital step in forensic DNA analysis to determine the optimal input
amount for DNA typing. A quantitative real-time polymerase chain reaction (qPCR) assay …

Comparison of nuclear DNA yield and STR typing success in Second World War petrous bones and metacarpals III

IZ Pajnič, J Inkret, T Zupanc, E Podovšovnik - Forensic Science International …, 2021 - Elsevier
DNA yield and STR typing success differ among skeletal element types and within individual
bones. Consequently, it is necessary to identify the skeletal elements and their intra-skeletal …

Improved reverse transcription-recombinase polymerase amplification assay for blood mRNA screening: comparison with one-step RT-qPCR assay

S Kubo, H Niimi, I Kitajima - Forensic Science International: Genetics, 2023 - Elsevier
Abstract mRNA profiling is effective for body fluid identification because of its sensitivity,
specificity, and multiplexing capability. Body fluid mRNA markers can typically be detected …