In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

Arp2/3 complex is an actin filament nucleation and branching machinery conserved in all
eukaryotes from yeast to human. Arp2/3 complex branched networks generate pushing …

Cortactin coactivates Arp2/3 complex synergistically with WASP-family nucleation-promoting
factors (NPFs) and stabilizes branched networks by linking Arp2/3 complex to F-actin. It is …

Arp2/3 complex nucleates branched actin filaments that provide pushing forces to drive
cellular processes such as lamellipodial protrusion and endocytosis. Arp2/3 complex is …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

We reconstructed the structure of actin filament branch junctions formed by fission yeast
Arp2/3 complex at 3.5 Å resolution from images collected by electron cryo-microscopy …

Actin-associated proteins (AAPs) act on monomeric globular actin (G-actin) and polymerized
filamentous actin (F-actin) to regulate their dynamics and architectures which ultimately …

Branched actin networks have emerged as major force-generating structures driving the
protrusions in various distinct cell types and processes, ranging from lamellipodia operating …

Integrative modeling enables structure determination of macromolecular complexes by
combining data from multiple experimental sources such as X-ray crystallography, electron …