Identifying the interactions established by a protein of interest can be a critical step in
understanding its function. This is especially true when an unknown protein of interest is …

The purification of recombinant proteins by affinity chromatography is one of the most
efficient strategies due to the high recovery yields and purity achieved. However, this is …

Chronic hepatitis B virus infection is a leading cause of cirrhosis and liver cancer,. Hepatitis
B virus encodes the regulatory HBx protein whose primary role is to promote transcription of …

Activation of the pseudokinase mixed lineage kinase domain-like (MLKL) upon its
phosphorylation by the protein kinase RIPK3 triggers necroptosis, a form of programmed cell …

Cellular organelles provide opportunities to relate biological mechanisms to disease. Here
we use affinity proteomics, genetics and cell biology to interrogate cilia: poorly understood …

Leucine-rich repeat kinase 2 (LRRK2) is a large, multidomain protein containing two
catalytic domains: a Ras of complex proteins (Roc) G-domain and a kinase domain …

Infection of mammalian cells with viruses activates NF-κB to induce the expression of
cytokines and chemokines and initiate an antiviral response. Here, we show that a vaccinia …

Immunomodulatory drugs (IMiDs), such as thalidomide and its derivatives lenalidomide and
pomalidomide, are key treatment modalities for hematologic malignancies, particularly …

Nephronophthisis is an autosomal recessive cystic kidney disease that leads to renal failure
in childhood or adolescence. Most NPHP gene products form molecular networks. Here we …

Phosphorylation of the pseudokinase mixed lineage kinase domain-like protein (MLKL) by
the protein kinase RIPK3 targets MLKL to the cell membrane, where it triggers necroptotic …