Biosynthesis and transport of nucleotide sugars for plant hemicellulose

W Zhang, W Qin, H Li, A Wu - Frontiers in plant science, 2021 - frontiersin.org
Hemicellulose is entangled with cellulose through hydrogen bonds and meanwhile acts as a
bridge for the deposition of lignin monomer in the secondary wall. Therefore, hemicellulose …

Rhamnose in plants-from biosynthesis to diverse functions

N Jiang, FM Dillon, A Silva, L Gomez-Cano… - Plant Science, 2021 - Elsevier
In plants, the deoxy sugar l-rhamnose is widely present as rhamnose-containing polymers in
cell walls and as part of the decoration of various specialized metabolites. Here, we review …

Functional characterization of a flavonol 3-O-rhamnosyltransferase and two UDP-rhamnose synthases from Hypericum monogynum

S Zhang, Y Wang, Z Cui, Q Li, L Kong, J Luo - Plant Physiology and …, 2023 - Elsevier
Rhamnosyltransferase (RT) and rhamnose synthase (Rhs) are the key enzymes that are
responsible for the biosynthesis of rhamnosides and UDP-l-rhamnose (UDP-Rha) in plants …

Identification and characterization of three flavonoid 3-O-glycosyltransferases from Epimedium koreanum Nakai

Y Lyu, S Liu, S Gao, J Zhou - Biochemical Engineering Journal, 2020 - Elsevier
Glycosylation of flavonoids is one of the most important modifications in flavonoid
biosynthesis pathways, which contributes to the biochemical activity as well as the solubility …

Elucidation of the Biosynthesis of Asiaticoside and Its Reconstitution in Yeast

X Zhao, W Wei, S Li, S Gao, S Liu… - ACS Sustainable …, 2024 - ACS Publications
Asiaticoside is a triterpenoid in Centella asiatica with wound healing and anti-inflammatory
activities. De novo production of asiaticoside remains incompletely resolved. To determine …

Isolation and characterization of a multifunctional flavonoid glycosyltransferase from Ornithogalum caudatum with glycosidase activity

S Yuan, S Yin, M Liu, JQ Kong - Scientific Reports, 2018 - nature.com
Glycosyltransferases (GTs) are bidirectional biocatalysts catalyzing the glycosylation of
diverse molecules. However, the extensive applications of GTs in glycosides formation are …

Enhancing UDP-Rhamnose Supply for Rhamnosylation of Flavonoids in Escherichia coli by Regulating the Modular Pathway and Improving NADPH Availability

N Gu, C Qiu, L Zhao, L Zhang, J Pei - Journal of Agricultural and …, 2020 - ACS Publications
UDP-rhamnose is the main type of sugar donor and endows flavonoids with special activity,
selectivity, and pharmacological properties by glycosylation. In this study, several UDP …

Construction of a novel UDP-rhamnose regeneration system by a two-enzyme reaction system and application in glycosylation of flavonoid

J Pei, A Chen, Q Sun, L Zhao, F Cao, F Tang - Biochemical engineering …, 2018 - Elsevier
UDP-rhamnose is synthesized by UDP-rhamnose synthase, but the reaction requires two
kinds of cofactors, NAD+ and NADPH. In this work, a cofactor self-sufficient UDP-rhamnose …

Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq

YJ Sun, JM He, JQ Kong - BMC plant biology, 2019 - Springer
Background Flavonol synthase (FLS) is the key enzyme responsible for the biosynthesis of
flavonols, the most abundant flavonoids, which have diverse pharmaceutical effects …

[HTML][HTML] The enzymatic biosynthesis of acylated steroidal glycosides and their cytotoxic activity

M Liu, JQ Kong - Acta pharmaceutica sinica B, 2018 - Elsevier
Herein we describe the discovery and functional characterization of a steroidal
glycosyltransferase (SGT) from Ornithogalum saundersiae and a steroidal glycoside …