Efficient and reproducible transgenesis facilitates and accelerates research using genetic
model organisms. Here, we describe a mo dular s afe-harbor t ransgene i nsertion (MosTI) …

I outline a streamlined method to insert large, single-copy transgenes into the
Caenorhabditis elegans genome using recombination-mediated cassette exchange (RMCE) …

High-throughput transgenesis using synthetic DNA libraries is a powerful method for
systematically exploring genetic function. Diverse synthesized libraries have been used for …

Sexual reproduction often leads to selection that favors the evolution of sex-limited traits or
sex-specific variation for shared traits. These sexual dimorphisms manifest due to sex …

Fluorescent proteins allow the expression of a gene and the behavior of its protein product
to be observed in living animals. The ability to create endogenous fluorescent protein tags …

Caenorhabditis elegans benefits from a large set of tools for genome manipulation. Yet, the
precise single-copy insertion of very large DNA constructs (> 10 kb) and the generation of …

Fertilization is a critical step in development, yet internal fertilization events are notoriously
difficult to visualize. Taking advantage of the calcium response that is a hallmark of sperm …

Calcium is an important signaling molecule during the oocyte-to-embryo transition (OET)
and early embryogenesis. The hermaphroditic nematode Caenorhabditis elegans provides …

Sexual reproduction is a complex process that contributes to differences between the sexes
and divergence between species. From a male's perspective, sexual selection can optimize …

Transgene expression in C. elegans is an important tool for understanding regulatory
elements (eg, promoters and enhancers), for controlling cells using optogenetic effector …