CRISPR–Cas is a widespread adaptive immune system in bacteria and archaea that
protects against viral infection by targeting specific invading nucleic acid sequences …

Among the multiple antiviral defense mechanisms found in prokaryotes, CRISPR-Cas
systems stand out as the only known RNA-programmed pathways for detecting and …

Summary Type I CRISPR-Cas systems typically rely on a two-step process to degrade DNA.
First, an RNA-guided complex named Cascade identifies the complementary DNA target …

Bacteria have diverse defenses against phages. In response, jumbo phages evade multiple
DNA-targeting defenses by protecting their DNA inside a nucleus-like structure. We …

Abstract CRISPR-associated Rossmann Fold (CARF) and S MODS-a ssociated and fused to
v arious e ffector d omains (SAVED) are key components of c yclic oligonucleotide-b ased a …

Type III CRISPR systems detect invading RNA, resulting in the activation of the enzymatic
Cas10 subunit. The Cas10 cyclase domain generates cyclic oligoadenylate (cOA) second …

Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-
nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded …

Abstract Type III CRISPR-Cas loci encode some of the most abundant, yet complex, immune
systems of prokaryotes. They are composed of a Cas10 complex that uses an RNA guide to …

This review summarizes the information about the history and future of the CRISPR/Cas9
method. Genome editing can be perceived as a group of technologies that allow scientists to …

Type III CRISPR–Cas systems make use of a multi-subunit effector complex to target foreign
(m) RNA transcripts complementary to the guide/CRISPR RNA (crRNA). Base-pairing of the …