Engineered bacteriophage-defence systems in bioprocessing

JM Sturino, TR Klaenhammer - Nature Reviews Microbiology, 2006 - nature.com
Bacteriophages (phages) have the potential to interfere with any industry that produces
bacteria as an end product or uses them as biocatalysts in the production of fermented …

Analysis, Characterization, and Loci of the tuf Genes in Lactobacillus and Bifidobacterium Species and Their Direct Application for Species Identification

M Ventura, C Canchaya, V Meylan… - Applied and …, 2003 - Am Soc Microbiol
We analyzed the tuf gene, encoding elongation factor Tu, from 33 strains representing 17
Lactobacillus species and 8 Bifidobacterium species. The tuf sequences were aligned and …

Targeted expression of anthrax protective antigen by Lactobacillus gasseri as an anthrax vaccine

M Mohamadzadeh, E Durmaz, M Zadeh… - Future …, 2010 - Taylor & Francis
Aim: Induction of protective immunity against pathogenic microbes, including Bacillus
anthracis, requires efficient vaccines that potentiate antibody avidity and increase T-cell …

Portable CRISPR-Cas9N System for Flexible Genome Engineering in Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus paracasei

YJ Goh, R Barrangou - Applied and Environmental Microbiology, 2021 - Am Soc Microbiol
Diverse Lactobacillus strains are widely used as probiotic cultures in the dairy and dietary
supplement industries, and specific strains, such as Lactobacillus acidophilus NCFM, have …

Identification and Cloning of gusA, Encoding a New β-Glucuronidase from Lactobacillus gasseriADH

WM Russell, TR Klaenhammer - Applied and environmental …, 2001 - Am Soc Microbiol
The gusA gene, encoding a new β-glucuronidase enzyme, has been cloned from
Lactobacillus gasseri ADH. This is the first report of a β-glucuronidase gene cloned from a …

A triggered-suicide system designed as a defense against bacteriophages

GM Djordjevic, DJ O'sullivan, SA Walker… - Journal of …, 1997 - Am Soc Microbiol
A novel bacteriophage protection system for Lactococcus lactis based on a genetic trap, in
which a strictly phage-inducible promoter isolated from the lytic phage phi31 is used to …

Expression of Antisense RNA Targeted against Streptococcus thermophilus Bacteriophages

JM Sturino, TR Klaenhammer - Applied and Environmental …, 2002 - Am Soc Microbiol
Antisense RNA complementary to a putative helicase gene (hel3. 1) of a cos-type
Streptococcus thermophilus bacteriophage was used to impede the proliferation of a …

Modification of Lactobacillus β-glucuronidase activity by random mutagenesis

MJ Callanan, WM Russell, TR Klaenhammer - Gene, 2007 - Elsevier
The Lactobacillus gasseri ADH β-glucuronidase gene, gusA, was cloned previously and
found to exhibit excellent activity in acidic pH ranges, with maximal activity at pH 5.0. In …

Molecular characterization of a phage-inducible middle promoter and its transcriptional activator from the lactococcal bacteriophage φ31

SA Walker, TR Klaenhammer - Journal of bacteriology, 1998 - Am Soc Microbiol
An inducible middle promoter from the lactococcal bacteriophage φ31 was isolated
previously by shotgun cloning an 888-bp fragment (P15A10) upstream of the β …

Genes Involved in Control of Galactose Uptake inLactobacillus brevis and Reconstitution of the Regulatory System in Bacillus subtilis

GM Djordjevic, JH Tchieu, MH Saier Jr - Journal of bacteriology, 2001 - Am Soc Microbiol
The heterofermentative lactic acid bacterium Lactobacillus brevis transports galactose and
the nonmetabolizable galactose analogue thiomethyl-β-galactoside (TMG) by a permease …