[HTML][HTML] Validation of optimal reference genes for quantitative real time PCR in muscle and adipose tissue for obesity and diabetes research

LJ Perez, L Rios, P Trivedi, K D'Souza, A Cowie… - Scientific reports, 2017 - nature.com
The global incidence of obesity has led to an increasing need for understanding the
molecular mechanisms that drive this epidemic and its comorbidities. Quantitative real-time …

[HTML][HTML] Identification of Optimal Reference Genes for Expression Analysis in Radish (Raphanus sativus L.) and Its Relatives Based on Expression Stability

M Duan, J Wang, X Zhang, H Yang, H Wang… - Frontiers in plant …, 2017 - frontiersin.org
Radish (Raphanus sativus) is an important cruciferous root crop with a close relationship to
Chinese cabbage (Brassica rapa). RT-qPCR is used extensively to evaluate the expression …

[HTML][HTML] Selection and validation of reference genes for quantitative real-time PCR analysis of development and tissue-dependent flower color formation in Cymbidium …

XM Dong, W Zhang, SB Zhang - International Journal of Molecular …, 2022 - mdpi.com
The development and tissue-dependent color formation of the horticultural plant results in
various color pattern flowers. Anthocyanins and carotenoids contribute to the red and yellow …

[HTML][HTML] Genomic characterization of WRKY transcription factors related to secoiridoid biosynthesis in Gentiana macrophylla

Y Yin, H Fu, F Mi, Y Yang, Y Wang, Z Li, Y He, Z Yue - BMC Plant Biology, 2024 - Springer
Gentiana macrophylla is one of Chinese herbal medicines in which 4 kinds of iridoids or
secoiridoids, such as loganic acid, sweroside, swertiamarin, and gentiopicroside, are …

[HTML][HTML] Identification and evaluation of reference genes for quantitative real-time PCR analysis in Polygonum cuspidatum based on transcriptome data

X Wang, Z Wu, W Bao, H Hu, M Chen, T Chai… - BMC plant biology, 2019 - Springer
Background Polygonum cuspidatum of the Polygonaceae family is a traditional medicinal
plant with many bioactive compounds that play important roles in human health and stress …

[HTML][HTML] The Orphan Crop Crassocephalum crepidioides Accumulates the Pyrrolizidine Alkaloid Jacobine in Response to Nitrogen Starvation

S Schramm, W Rozhon, AN Adedeji-Badmus… - Frontiers in Plant …, 2021 - frontiersin.org
Crassocephalum crepidioides is an African orphan crop that is used as a leafy vegetable
and medicinal plant. Although it is of high regional importance in Sub-Saharan Africa, the …

[HTML][HTML] Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L

Y Wang, Y Zhang, Q Liu, H Tong, T Zhang, C Gu… - Scientific Reports, 2021 - nature.com
Iris germanica L. is a perennial herbaceous plant that has been widely cultivated worldwide
and is popular for its elegant and vibrantly colorful flowers. Selection of appropriate …

Gentiana straminea Maxim. polysaccharide decolored via high-throughput graphene-based column and its anti-inflammatory activity

S Shi, G Wang, J Liu, S Liu, Q Xu, X Lan, J Feng… - International Journal of …, 2021 - Elsevier
Gentiana straminea Maxim. exhibits various biological activities. However, the purification
and functions of polysaccharides in Gentiana straminea Maxim. have never been reported …

[HTML][HTML] Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli

H Sun, X Jiang, M Sun, H Cong, F Qiao - Plant Methods, 2019 - Springer
Background Reverse transcription quantitative real-time PCR (RT-qPCR) is a widely used
approach for investigating gene expression levels in plants because of its high …

[HTML][HTML] Validation of Reference Genes for Quantitative Real-Time PCR Normalization in Ananas comosus var. bracteatus During Chimeric Leaf Development and …

M Mao, Y Xue, Y He, X Zhou, H Hu, J Liu, L Feng… - Frontiers in …, 2021 - frontiersin.org
Reverse transcription quantitative real-time PCR (RT-qPCR) is a common way to study gene
regulation at the transcriptional level due to its sensibility and specificity, but it needs …