Abstract BACKGROUND Digital PCR (dPCR) is an increasingly popular manifestation of
PCR that offers a number of unique advantages when applied to preclinical research …

Successful treatment of cancer depends on early diagnosis and effective monitoring of
patients' response to therapy. Traditional tools based on tumor biopsies lack the sensitivity …

Environmental DNA (eDNA) is used to detect biodiversity by the capture, extraction, and
identification of DNA shed to the environment. However, eDNA capture and extraction …

There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …

BACKGROUND There is great interest in circulating microRNAs (miRNAs) as disease
biomarkers. Translating promising miRNAs into validated clinical tests requires the …

Molecular methods are being increasingly applied to detect, quantify and study microbial
populations in food or during food processes. Among these methods, PCR-based …

In the analysis of quantitative PCR (qPCR) data, the quantification cycle (Cq) indicates the
position of the amplification curve with respect to the cycle axis. Because Cq is directly …

The polymerase chain reaction (PCR) has matured from a labour-and time-intensive, low
throughput qualitative gel-based technique to an easily automated, rapid, high throughput …

RNA transcripts such as mRNA or microRNA are frequently used as biomarkers to
determine disease state or response to therapy. Reverse transcription (RT) in combination …

This paper assesses the quantitative resolution of qPCR using copy number variation (CNV)
as a paradigm. An error model is developed for real-time qPCR data showing how the …