[HTML][HTML] Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics
Pseudomonas aeruginosa (P. aeruginosa) is a Gram-negative opportunistic pathogen that
infects patients with cystic fibrosis, burn wounds, immunodeficiency, chronic obstructive …
infects patients with cystic fibrosis, burn wounds, immunodeficiency, chronic obstructive …
Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
[HTML][HTML] Cell-free gene expression
D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …
specific applications in protein synthesis and labelling for structural biology and proteomics …
Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies
Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-
associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have …
associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have …
Discovery of widespread type I and type V CRISPR-Cas inhibitors
Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile
genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to …
genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to …
The development of a fluorescence/colorimetric biosensor based on the cleavage activity of CRISPR-Cas12a for the detection of non-nucleic acid targets
Y Wang, Y Peng, S Li, D Han, S Ren, K Qin… - Journal of Hazardous …, 2023 - Elsevier
Nano-biosensors are of great significance for the analysis and detection of important
biological targets. Surprisingly, the CRISPR-Cas12a system not only provides us with …
biological targets. Surprisingly, the CRISPR-Cas12a system not only provides us with …
[HTML][HTML] Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA
O Dmytrenko, GC Neumann, T Hallmark, DJ Keiser… - Nature, 2023 - nature.com
Bacterial abortive-infection systems limit the spread of foreign invaders by shutting down or
killing infected cells before the invaders can replicate 1, 2. Several RNA-targeting CRISPR …
killing infected cells before the invaders can replicate 1, 2. Several RNA-targeting CRISPR …
Inhibitors of bacterial immune systems: discovery, mechanisms and applications
D Mayo-Muñoz, R Pinilla-Redondo… - Nature Reviews …, 2024 - nature.com
To contend with the diversity and ubiquity of bacteriophages and other mobile genetic
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …
Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins
CRISPR loci and Cas proteins provide adaptive immunity in prokaryotes against invading
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
Anti-CRISPRs: protein inhibitors of CRISPR-Cas systems
AR Davidson, WT Lu, SY Stanley… - Annual review of …, 2020 - annualreviews.org
Clustered regularly interspaced short palindromic repeats (CRISPR) together with their
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …