Celastrol is a promising bioactive compound isolated from Tripterygium wilfordii and has
been shown to possess many encouraging preclinical applications. However, the celastrol …

Key message We cloned two squalene epoxidases and five oxidosqualene cyclases, and
identified their function using CRISPR/Cas9 tool and yeast heterologous expression …

Friedelin, the most rearranged pentacyclic triterpene, also exhibits remarkable
pharmacological and anti-insect activities. In particular, celastrol with friedelin as the …

Triterpenoids are a subgroup of terpenoids and have wide applications in the food,
cosmetics, and pharmaceutical industries. The heterologous production of various …

Main conclusion An oxidosqualene cyclase (PdFRS) from Populus davidiana was
characterized as a monofunctional friedelin synthase by its heterologous expression in yeast …

Among the biologically active triterpenes, friedelin has the most-rearranged structure
produced by the oxidosqualene cyclases and is the only one containing a cetonic group. In …

Triterpenoids represent a diverse group of phytochemicals that are widely distributed in the
plant kingdom and have many biological activities. The heterologous production of …

1712 2004 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim DOI: 10.1002/cbic. 200400079
ChemBioChem 2004, 5, 1712–1715 uct profiles in triterpene cyclase enzymes.[3–16] For …

Sesterterpenoids are a group of terpenoid natural products that are primarily biosynthesized
via cyclization of the C25 linear substrate geranylfarnesyl pyrophosphate (GFPP). Although …

Tripterygium wilfordii is a perennial woody liana medicinal plant with several crucial
biological activities. Although studies on tissue culture have previously been conducted …