Systematic exploration of essential yeast gene function with temperature-sensitive mutants
Z Li, FJ Vizeacoumar, S Bahr, J Li, J Warringer… - Nature …, 2011 - nature.com
Conditional temperature-sensitive (ts) mutations are valuable reagents for studying essential
genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 …
genes in the yeast Saccharomyces cerevisiae. We constructed 787 ts strains, covering 497 …
[HTML][HTML] Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae
S Ben-Aroya, C Coombes, T Kwok, KA O'Donnell… - Molecular cell, 2008 - cell.com
The Saccharomyces cereivisiae gene deletion project revealed that approximately 20% of
yeast genes are required for viability. The analysis of essential genes traditionally relies on …
yeast genes are required for viability. The analysis of essential genes traditionally relies on …
Why are there still over 1000 uncharacterized yeast genes?
L Pena-Castillo, TR Hughes - Genetics, 2007 - academic.oup.com
The yeast genetics community has embraced genomic biology, and there is a general
understanding that obtaining a full encyclopedia of functions of the∼ 6000 genes is a …
understanding that obtaining a full encyclopedia of functions of the∼ 6000 genes is a …
An integrated approach for finding overlooked genes in yeast
We report here the discovery of 137 previously unappreciated genes in yeast through a
widely applicable and highly scalable approach integrating methods of gene-trapping …
widely applicable and highly scalable approach integrating methods of gene-trapping …
[HTML][HTML] Exploration of essential gene functions via titratable promoter alleles
Nearly 20% of yeast genes are required for viability, hindering genetic analysis with
knockouts. We created promoter-shutoff strains for over two-thirds of all essential yeast …
knockouts. We created promoter-shutoff strains for over two-thirds of all essential yeast …
A comprehensive strategy enabling high-resolution functional analysis of the yeast genome
Functional genomic studies in Saccharomyces cerevisiae have contributed enormously to
our understanding of cellular processes. Their full potential, however, has been hampered …
our understanding of cellular processes. Their full potential, however, has been hampered …
Activity motifs reveal principles of timing in transcriptional control of the yeast metabolic network
Significant insight about biological networks arises from the study of network motifs—overly
abundant network subgraphs,—but such wiring patterns do not specify when and how …
abundant network subgraphs,—but such wiring patterns do not specify when and how …
Intrinsic biocontainment: multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes
Biocontainment may be required in a wide variety of situations such as work with pathogens,
field release applications of engineered organisms, and protection of intellectual properties …
field release applications of engineered organisms, and protection of intellectual properties …
Systematic functional analysis of the yeast genome
The genome sequence of the yeast Saccharomyces cerevisiae has provided the first
complete inventory of the working parts of a eukaryotic cell. The challenge is now to discover …
complete inventory of the working parts of a eukaryotic cell. The challenge is now to discover …
Large Scale Identification of Genes Involved in Cell Surface Biosynthesis and Architecture in Saccharomyces cerevisiae
M Lussier, AM White, J Sheraton, T di Paolo… - Genetics, 1997 - academic.oup.com
The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell
function and enables genome-wide screens for genes involved in cellular processes. We …
function and enables genome-wide screens for genes involved in cellular processes. We …