The catalytic null mutant of the Cas9 endonuclease from the bacterial CRISPR immune
system, known as dCas9, can be guided by a small RNA to bind DNA sequences of interest …

Functional genetic screening is an important method that has been widely used to explore
the biological processes and functional annotation of genetic elements. CRISPR/Cas …

Prokaryotes have evolved several defence mechanisms to protect themselves from viral
predators. Clustered regularly interspaced short palindromic repeats (CRISPR) and their …

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR
associated (cas) genes conform the CRISPR-Cas systems of various bacteria and archaea …

Abstract CRISPR-Cas12a (Cpf1) is a bacterial RNA-guided nuclease that cuts double-
stranded DNA (dsDNA) at sites specified by a CRISPR RNA (crRNA) guide. Additional …

The Cas9 endonuclease is the central component of the Type II CRISPR/Cas system, a
prokaryotic adaptive restriction system against invading nucleic acids, such as those …

Used widely for genome editing, CRISPR-Cas enzymes provide RNA-guided immunity to
microbes by targeting foreign nucleic acids for cleavage. We show here that the native …

High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to
decipher gene functions and genetic interactions. Here we use a genome-wide library of …

ABSTRACT CRISPR interference (CRISPRi) is a powerful new tool used in different
organisms that provides a fast, specific, and reliable way to knock down gene expression …

Few discoveries transform a discipline overnight, but biologists today can manipulate cells in
ways never possible before, thanks to a peculiar form of prokaryotic adaptive immunity …