Cryopreservation of human adipose-derived stem cells in combination with trehalose and reversible electroporation

B Dovgan, A Barlič, M Knežević, D Miklavčič - The Journal of membrane …, 2017 - Springer
New cryopreservation approaches for medically applicable cells are of great importance in
clinical medicine. Current protocols employ the use of dimethyl sulfoxide (DMSO), which is …

Intracellular delivery of trehalose renders mesenchymal stromal cells viable and immunomodulatory competent after cryopreservation

B Dovgan, D Miklavčič, M Knežević, J Zupan, A Barlič - Cytotechnology, 2021 - Springer
Trehalose is a nontoxic disaccharide and a promising cryoprotection agent for medically
applicable cells. In this study, the efficiency of combining trehalose with reversible …

The combination of trehalose and glycerol: an effective and non-toxic recipe for cryopreservation of human adipose-derived stem cells

TY Zhang, PC Tan, Y Xie, XJ Zhang, PQ Zhang… - Stem Cell Research & …, 2020 - Springer
Background Adipose-derived stem cells (ADSCs) promote tissue regeneration and repair.
Cryoprotective agents (CPAs) protect cells from cryodamage during cryopreservation. Safe …

Intracellular delivery of trehalose for cell banking

S Stewart, X He - Langmuir, 2018 - ACS Publications
Advances in stem cell technology and regenerative medicine have underscored the need for
effective banking of living cells. Cryopreservation, using very low temperatures to achieve …

Cryopreservation of stem cells using trehalose: evaluation of the method using a human hematopoietic cell line

SS Buchanan, SA Gross, JP Acker, M Toner… - Stem cells and …, 2004 - liebertpub.com
While stem cell cryopreservation methods have been optimized using dimethylsulfoxide
(DMSO), the established techniques are not optimal when applied to unfertilized human …

[HTML][HTML] Amphipathic polymer-mediated uptake of trehalose for dimethyl sulfoxide-free human cell cryopreservation

DMC Sharp, A Picken, TJ Morris, CJ Hewitt… - Cryobiology, 2013 - Elsevier
For stem cell therapy to become a routine reality, one of the major challenges to overcome is
their storage and transportation. Currently this is achieved by cryopreserving cells utilising …

Cold-responsive nanoparticle enables intracellular delivery and rapid release of trehalose for organic-solvent-free cryopreservation

Y Zhang, H Wang, S Stewart, B Jiang, W Ou, G Zhao… - Nano …, 2019 - ACS Publications
Conventional cryopreservation of mammalian cells requires the use of toxic organic solvents
(eg, dimethyl sulfoxide) as cryoprotectants. Consequently, the cryopreserved cells must …

Loading trehalose into red blood cells by electroporation and its application in freeze-drying

X Zhou, J Yuan, J Liu, B Liu - Cryoletters, 2010 - ingentaconnect.com
Freeze-drying is one potentially ideal method for long-term storage of red blood cells
(RBCs). Intracellular trehalose is believed to be an effective protectant to stabilize cells …

[HTML][HTML] Trehalose to cryopreserve human pluripotent stem cells

A Ntai, A La Spada, P De Blasio, I Biunno - Stem Cell Research, 2018 - Elsevier
The successful exploitation of human pluripotent stem cells (hPSCs) for research,
translational or commercial reasons requires the implementation of a simple and efficient …

Cryopreservation characteristics of adipose‐derived stem cells: maintenance of differentiation potential and viability

BC Goh, S Thirumala, G Kilroy… - Journal of tissue …, 2007 - Wiley Online Library
With the emergence of regenerative medicine, many researchers have turned to fat tissue as
a source of adipose‐derived stem cells (ASCs). Because freshly collected adipose tissue is …