Cryopreservation of human adipose-derived stem cells in combination with trehalose and reversible electroporation
B Dovgan, A Barlič, M Knežević, D Miklavčič - The Journal of membrane …, 2017 - Springer
New cryopreservation approaches for medically applicable cells are of great importance in
clinical medicine. Current protocols employ the use of dimethyl sulfoxide (DMSO), which is …
clinical medicine. Current protocols employ the use of dimethyl sulfoxide (DMSO), which is …
Intracellular delivery of trehalose renders mesenchymal stromal cells viable and immunomodulatory competent after cryopreservation
B Dovgan, D Miklavčič, M Knežević, J Zupan, A Barlič - Cytotechnology, 2021 - Springer
Trehalose is a nontoxic disaccharide and a promising cryoprotection agent for medically
applicable cells. In this study, the efficiency of combining trehalose with reversible …
applicable cells. In this study, the efficiency of combining trehalose with reversible …
The combination of trehalose and glycerol: an effective and non-toxic recipe for cryopreservation of human adipose-derived stem cells
TY Zhang, PC Tan, Y Xie, XJ Zhang, PQ Zhang… - Stem Cell Research & …, 2020 - Springer
Background Adipose-derived stem cells (ADSCs) promote tissue regeneration and repair.
Cryoprotective agents (CPAs) protect cells from cryodamage during cryopreservation. Safe …
Cryoprotective agents (CPAs) protect cells from cryodamage during cryopreservation. Safe …
Intracellular delivery of trehalose for cell banking
Advances in stem cell technology and regenerative medicine have underscored the need for
effective banking of living cells. Cryopreservation, using very low temperatures to achieve …
effective banking of living cells. Cryopreservation, using very low temperatures to achieve …
Cryopreservation of stem cells using trehalose: evaluation of the method using a human hematopoietic cell line
While stem cell cryopreservation methods have been optimized using dimethylsulfoxide
(DMSO), the established techniques are not optimal when applied to unfertilized human …
(DMSO), the established techniques are not optimal when applied to unfertilized human …
[HTML][HTML] Amphipathic polymer-mediated uptake of trehalose for dimethyl sulfoxide-free human cell cryopreservation
DMC Sharp, A Picken, TJ Morris, CJ Hewitt… - Cryobiology, 2013 - Elsevier
For stem cell therapy to become a routine reality, one of the major challenges to overcome is
their storage and transportation. Currently this is achieved by cryopreserving cells utilising …
their storage and transportation. Currently this is achieved by cryopreserving cells utilising …
Cold-responsive nanoparticle enables intracellular delivery and rapid release of trehalose for organic-solvent-free cryopreservation
Conventional cryopreservation of mammalian cells requires the use of toxic organic solvents
(eg, dimethyl sulfoxide) as cryoprotectants. Consequently, the cryopreserved cells must …
(eg, dimethyl sulfoxide) as cryoprotectants. Consequently, the cryopreserved cells must …
Loading trehalose into red blood cells by electroporation and its application in freeze-drying
X Zhou, J Yuan, J Liu, B Liu - Cryoletters, 2010 - ingentaconnect.com
Freeze-drying is one potentially ideal method for long-term storage of red blood cells
(RBCs). Intracellular trehalose is believed to be an effective protectant to stabilize cells …
(RBCs). Intracellular trehalose is believed to be an effective protectant to stabilize cells …
[HTML][HTML] Trehalose to cryopreserve human pluripotent stem cells
A Ntai, A La Spada, P De Blasio, I Biunno - Stem Cell Research, 2018 - Elsevier
The successful exploitation of human pluripotent stem cells (hPSCs) for research,
translational or commercial reasons requires the implementation of a simple and efficient …
translational or commercial reasons requires the implementation of a simple and efficient …
Cryopreservation characteristics of adipose‐derived stem cells: maintenance of differentiation potential and viability
BC Goh, S Thirumala, G Kilroy… - Journal of tissue …, 2007 - Wiley Online Library
With the emergence of regenerative medicine, many researchers have turned to fat tissue as
a source of adipose‐derived stem cells (ASCs). Because freshly collected adipose tissue is …
a source of adipose‐derived stem cells (ASCs). Because freshly collected adipose tissue is …