CRISPR-Cas12a-mediated label-free electrochemical aptamer-based sensor for SARS-CoV-2 antigen detection

N Liu, R Liu, J Zhang - Bioelectrochemistry, 2022 - Elsevier
Serological antigen testing has emerged as an important diagnostic paradigm in COVID-19,
but often suffers from potential cross-reactivity. To address this limitation, we herein report a …

CRISPR/Cas12a-Derived electrochemical aptasensor for ultrasensitive detection of COVID-19 nucleocapsid protein

C Han, W Li, Q Li, W Xing, H Luo, H Ji, X Fang… - Biosensors and …, 2022 - Elsevier
Fast, affordable, portable, and sensitive technology to detect COVID-19 is critical to address
the current outbreak. Here, we present a CRISPR/Cas12a-derived electrochemical …

Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode

L Wu, X Wang, C Wu, X Cao, T Tang, H Huang… - Analytica Chimica …, 2022 - Elsevier
Early and accurate diagnosis of SARS-CoV-2 was crucial for COVID-19 control and urgently
required ultra-sensitive and rapid detection methods. CRISPR-based detection systems …

CRISPR-Cas12a-empowered electrochemical biosensor for rapid and ultrasensitive detection of SARS-CoV-2 delta variant

C Wu, Z Chen, C Li, Y Hao, Y Tang, Y Yuan, L Chai… - Nano-Micro Letters, 2022 - Springer
Abstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method …

Electrochemical biosensor for nucleic acid amplification-free and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA via …

W Heo, K Lee, S Park, KA Hyun, HI Jung - Biosensors and Bioelectronics, 2022 - Elsevier
The outbreak of the COVID-19 pandemic has led to millions of fatalities worldwide. For
preventing epidemic transmission, rapid and accurate virus detection methods to early …

Ultrasensitive and amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor powered by CRISPR/Cas13a

L Kashefi-Kheyrabadi, HV Nguyen, A Go, MH Lee - Bioelectrochemistry, 2023 - Elsevier
This study proposed a CRISPR/Cas13a-powered electrochemical multiplexed biosensor for
detecting SARS-CoV-2 RNA strands. Current SARS-CoV-2 diagnostic methods, such as …

Customization of aptamer to develop CRISPR/Cas12a-derived ultrasensitive biosensor

W Xing, Q Li, C Han, D Sun, Z Zhang, X Fang, Y Guo… - Talanta, 2023 - Elsevier
The CRISPR/Cas systems have provided wide biosensing applications. Particularly, the
aptamer-involved CRISPR/Cas sensor system powerfully expanded to non-nucleic-acid …

CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants

Z Chen, C Wu, Y Yuan, Z Xie, T Li, H Huang… - Journal of …, 2023 - Springer
Since the end of 2019, a highly contagious disease caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) has deprived numerous lives worldwide, called …

Highly sensitive electrochemical aptasensor for SARS-CoV-2 antigen detection based on aptamer-binding induced multiple hairpin assembly signal amplification

J Xue, Y Li, J Liu, Z Zhang, R Yu, Y Huang, C Li… - Talanta, 2022 - Elsevier
In this work, a brief electrochemical aptasensor was developed for highly sensitive detection
of SARS-CoV-2 antigen utilizing an aptamer-binding induced multiple hairpin assembly …

An electrochemical impedance spectroscopy-based aptasensor for the determination of SARS-CoV-2-RBD using a carbon nanofiber–gold nanocomposite modified …

M Amouzadeh Tabrizi, P Acedo - Biosensors, 2022 - mdpi.com
Worldwide, human health is affected by severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2). Hence, the fabrication of the biosensors to diagnose SARS-CoV-2 is critical …