Despite remarkable recent achievements of immunotherapy strategies in cancer treatment,
clinical responses remain limited to subsets of patients. Predictive markers of disease …

A robust understanding of the tumor immune environment has important implications for
cancer diagnosis, prognosis, research, and immunotherapy. Traditionally …

Continued developments in immuno-oncology require an increased understanding of the
mechanisms of cancer immunology. The immunoprofiling analysis of tissue samples from …

Immune profiling is becoming a vital tool for identifying predictive and prognostic markers for
translational studies. The study of the tumor microenvironment (TME) in paraffin tumor …

Multiplexed platforms for multiple epitope detection have emerged in the last years as very
powerful tools to study tumor tissues. These revolutionary technologies provide important …

Our understanding of the biology and management of human disease has undergone a
remarkable evolution in recent decades. Improved understanding of the roles of complex …

Multiplex immunofluorescence is a powerful tool for the simultaneous detection of tissue‐
based biomarkers, revolutionising traditional immunohistochemistry. The Opal methodology …

Multiplexed imaging platforms to simultaneously detect multiple epitopes in the same tissue
section emerged in the last years as very powerful tools to study tumor immune contexture …

Biomarker assessments of tumor specimens is widely used in cancer research to audit tumor
cell intrinsic as well as tumor cell extrinsic features including the diversity of immune …

Here, we describe a multiplexed immunohistochemical platform with computational image
processing workflows, including image cytometry, enabling simultaneous evaluation of 12 …