RB-TnSeq identifies genetic targets for improved tolerance of Pseudomonas putida towards compounds relevant to lignin conversion
Lignin-derived mixtures intended for bioconversion commonly contain high concentrations
of aromatic acids, aliphatic acids, and salts. The inherent toxicity of these chemicals places a …
of aromatic acids, aliphatic acids, and salts. The inherent toxicity of these chemicals places a …
Adaptive laboratory evolution of Pseudomonas putida KT2440 improves p-coumaric and ferulic acid catabolism and tolerance
ET Mohamed, AZ Werner, D Salvachúa… - Metabolic engineering …, 2020 - Elsevier
Pseudomonas putida KT2440 is a promising bacterial chassis for the conversion of lignin-
derived aromatic compound mixtures to biofuels and bioproducts. Despite the inherent …
derived aromatic compound mixtures to biofuels and bioproducts. Despite the inherent …
Systems biology-guided biodesign of consolidated lignin conversion
Lignin is the second most abundant biopolymer on the earth, yet its utilization for fungible
products is complicated by its recalcitrant nature and remains a major challenge for …
products is complicated by its recalcitrant nature and remains a major challenge for …
Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida
As the most abundant natural aromatic resource, lignin valorization will contribute to a
feasible biobased economy. Recently, biological lignin valorization has been advocated …
feasible biobased economy. Recently, biological lignin valorization has been advocated …
[PDF][PDF] Multiplexed fitness profiling by RB-TnSeq elucidates pathways for lignin-related aromatic catabolism in Sphingobium sp. SYK-6
A Bleem, R Kato, ZA Kellermyer, R Katahira… - Cell Reports, 2023 - cell.com
Bioconversion of lignin-related aromatic compounds relies on robust catabolic pathways in
microbes. Sphingobium sp. SYK-6 (SYK-6) is a well-characterized aromatic catabolic …
microbes. Sphingobium sp. SYK-6 (SYK-6) is a well-characterized aromatic catabolic …
Current Status of Pseudomonas putida Engineering for Lignin Valorization
S Lee, JH Sohn, JH Bae, SC Kim, BH Sung - … and Bioprocess Engineering, 2020 - Springer
Lignin, a complex aromatic polymer, is a structural component of plant biomass. It
decomposes with difficulty because of its rigidity properties, however, lignin valorization is …
decomposes with difficulty because of its rigidity properties, however, lignin valorization is …
Metabolism of syringyl lignin-derived compounds in Pseudomonas putida enables convergent production of 2-pyrone-4, 6-dicarboxylic acid
S Notonier, AZ Werner, E Kuatsjah, L Dumalo… - Metabolic …, 2021 - Elsevier
Valorization of lignin, an abundant component of plant cell walls, is critical to enabling the
lignocellulosic bioeconomy. Biological funneling using microbial biocatalysts has emerged …
lignocellulosic bioeconomy. Biological funneling using microbial biocatalysts has emerged …
Engineering Pseudomonas putida for improved utilization of syringyl aromatics
Lignin is a largely untapped source for the bioproduction of value‐added chemicals.
Pseudomonas putida KT2440 has emerged as a strong candidate for bioprocessing of lignin …
Pseudomonas putida KT2440 has emerged as a strong candidate for bioprocessing of lignin …
Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin
CW Johnson, GT Beckham - Metabolic engineering, 2015 - Elsevier
Lignin represents an untapped feedstock for the production of fuels and chemicals, but its
intrinsic heterogeneity makes lignin valorization a significant challenge. In nature, many …
intrinsic heterogeneity makes lignin valorization a significant challenge. In nature, many …
Response of Pseudomonas putida to Complex, Aromatic‐Rich Fractions from Biomass
MR Park, Y Chen, M Thompson, VT Benites… - …, 2020 - Wiley Online Library
There is strong interest in the valorization of lignin to produce valuable products; however,
its structural complexity has been a conversion bottleneck. Chemical pretreatment liberates …
its structural complexity has been a conversion bottleneck. Chemical pretreatment liberates …
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