Oviduct fluid extracellular vesicles regulate polyspermy during porcine in vitro fertilisation

AS Alcântara-Neto, M Fernandez-Rufete… - Reproduction …, 2020 - CSIRO Publishing
High polyspermy is one of the major limitations of porcine invitro fertilisation (IVF). The
addition of oviductal fluid (OF) during IVF reduces polyspermy without decreasing the …

Porcine oviductal extracellular vesicles interact with gametes and regulate sperm motility and survival

AS Alcântara-Neto, L Schmaltz, E Caldas, MC Blache… - Theriogenology, 2020 - Elsevier
Once in the female reproductive tract, spermatozoa undergo several modifications to acquire
their complete fertilizing ability. Interactions between the oviductal fluid (OF) and gametes …

[HTML][HTML] Combination of oviduct fluid and heparin to improve monospermic zygotes production during porcine in vitro fertilization

RITP Batista, LN Moro, E Corbin, C Alminana… - Theriogenology, 2016 - Elsevier
In vivo, the oviduct provides appropriate microenvironment conditions for monospermic
fertilization and early embryo development. In addition, glycosaminoglycans such as …

[HTML][HTML] Timing of oviductal fluid collection, steroid concentrations, and sperm preservation method affect porcine in vitro fertilization efficiency

L Ballester, J Romero-Aguirregomezcorta… - Fertility and sterility, 2014 - Elsevier
Objective To determine optimal conditions for the inclusion of oviductal fluid (OF) in IVF
protocols. Design Experimental prospective study. Setting Mammalian reproduction …

Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality

CLV Leal, K Cañón-Beltrán, YN Cajas, M Hamdi… - Journal of Animal …, 2022 - Springer
Background In vitro production of bovine embryos is a well-established technology, but the
in vitro culture (IVC) system still warrants improvements, especially regarding embryo …

The role of the oviduct and extracellular vesicles during early embryo development in bovine

NM Bastos, JG Ferst, RS Goulart… - Animal …, 2022 - SciELO Brasil
The oviduct is an important reproductive structure that connects the ovary to the uterus and
takes place to important events such as oocyte final maturation, fertilization and early …

Effects of oviductal fluid on sperm penetration and cortical granule exocytosis during fertilization of pig oocytes in vitro

NH Kim, H Funahashi, LR Abeydeera, SJ Moon… - …, 1996 - rep.bioscientifica.com
The effects of oviductal fluid on sperm penetration and cortical granule exocytosis in pigs
were examined. Cortical granule exocytosis in oocytes matured in vivo and in vitro was …

Dynamic profile of EVs in porcine oviductal fluid during the periovulatory period

I Laezer, SE Palma-Vera, F Liu, M Frank… - …, 2020 - rep.bioscientifica.com
In mammals, around the time of ovulation, the hormonal profile dynamically changes in
synchrony with reproductive events occurring in the oviduct, that is, sperm arrival …

Changes induced in the bovine zona pellucida by ovine and bovine oviducts

RT Duby, JL Hill, D O'Callaghan, EW Overstrom… - Theriogenology, 1997 - infona.pl
The block to polyspermy is not well defined in cattle. While dispersion of and release of the
contents of the cortical granules (CG) occurs there is a high incidence of polyspermy in …

Oviductal extracellular vesicles enhance porcine in vitro embryo development by modulating the embryonic transcriptome

AS de Alcântara-Neto, C Cuello, R Uzbekov… - Biomolecules, 2022 - mdpi.com
Oviductal extracellular vesicles (oEVs) have been identified as important components of the
oviductal fluid (OF) and have been pointed to as key modulators of gamete/embryo-maternal …