Sindbis virus can infect a broad range of insect and vertebrate cell types due to the
widespread distribution of the cellular receptor for the virus. The development of Sindbis …

Sindbis virus and other alphavirus gene expression vectors have recently been used to
express and study the functions of proteins and RNA, to evaluate classical vaccine and …

Sindbis virus can infect a broad range of insect and vertebrate cell types. The ability to
restrict tissue tropism and target virus infection to specific cell types would expand the …

Several DNA-based Sindbis virus vectors were constructed to investigate the feasibility and
potential applications for initiating the virus life cycle in cells transfected directly with plasmid …

Alphavirus vectors are being developed for possible human vaccine and gene therapy
applications. We have sought to advance this field by devising DNA-based vectors and …

The ability to target antigen-presenting cells with vectors encoding desired antigens holds
the promise of potent prophylactic and therapeutic vaccines for infectious diseases and …

Alphavirus-based expression vectors commonly use a duplicated 26S promoter to drive
expression of a foreign gene. Here we describe an expression strategy in which the foreign …

The prototype of the Alphaviruses, Sindbis virus, has a broad host range. In nature, Sindbis
virus shuttles from an insect vector to a vertebrate host and back to the insect vector in a …

Infection of vertebrate cells with alphaviruses normally leads to prodigious expression of
virus-encoded genes and a dramatic inhibition of host protein synthesis. Recombinant …

Alphaviruses are RNA enveloped viruses that are proving their value as expression vectors.
They are particularly well-suited for this role as they are easily and quickly engineered and …