A Shewanella expression system has been used for an overproduction of c-type multiheme
proteins. The proteins were exported to the periplasmic space for the maturation. Since the …

The sequence of the omcA gene, which encodes a decaheme cytochrome c that is localized
to the outer membrane (OM) of Shewanella putrefaciens MR-1, was determined. The 2202 …

The genes of tetraheme cytochrome c3 and hexadecaheme high-molecular-weight
cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in …

The cytochrome b5 of the body wall of adult Ascaris suum, a porcine parasitic nematode, is a
novel type of cytochrome b5. It is a soluble protein that lacks the COOH-terminal membrane …

Cytochrome c 3 from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the
facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and …

Figure 1. Absorption spectra of oxidized and reduced of DVU3171. The protocols to isolate
MtrA or DVU3171 are nearly the same. Shewanella strains with the construct containing the …

We have functionally produced the outer membrane cytochrome OmcA from Shewanella
oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is …

Abstract The Tn 5-based transposon Tn 5-KIL3 (Miksch et al. 1997c) bearing the kil gene of
the ColE1 plasmid of Escherichia coli, which mediates controlled export of periplasmic …

Two abundant, low-redox-potential cytochromes c were purified from the facultative
anaerobe Shewanella oneidensis strain MR1 grown anaerobically with fumarate. The small …

A simple approach is described for the production and purification of proteins in
Pseudomonas syringae. The strategy involves the use of the tac promoter, the …