In vitro production of bovine embryos and their application for embryo transfer

H Hoshi - Theriogenology, 2003 - Elsevier
This review introduces newly developed serum-free media (IVD101 and IVMD101), that are
effective for producing high yields of transferable embryos of good quality from in vitro …

Evaluation of bovine embryos produced in high performance serum-free media

H Abe, H Hoshi - Journal of Reproduction and Development, 2003 - jstage.jst.go.jp
AEstract. This review evaluates the quality of bovine embryos developed from in vitro
matured (IVM) and fertilized (IVf) oocytes cultured in either serum free or serum containing …

Development of in vitro matured/in vitro fertilized bovine embryos into morulae and blastocysts in defined culture media

BD Bavister, TA Rose-Hellekant, T Pinyopummintr - Theriogenology, 1992 - Elsevier
The techniques of in vitro maturation (IVM) and in vitro fertilization (IVF) of bovine oocytes,
with development of the resulting embryos to advanced preimplantation stages in vitro, have …

A serum-free culture system for efficient in vitro production of bovine blastocysts with improved viability after freezing and thawing

S Yamashita, H Abe, T Itoh, T Satoh, H Hoshi - Cytotechnology, 1999 - Springer
The aim of this study was to evaluate whether two completely serum-free media (IVMD101
and IVD101) could improve the yield and quality of bovine blastocysts from in vitro matured …

Set up of a serum-free culture system for bovine embryos: embryo development and quality before and after transient transfer

F George, C Daniaux, G Genicot, B Verhaeghe… - Theriogenology, 2008 - Elsevier
It is well known that serum in culture medium negatively affects blastocyst quality. The
objective of this work was to develop and test a serum-free culture medium which could …

In vitro culture and evaluation of embryos for production of high quality bovine embryos

H Abe, H Shiku, S Aoyagi, H Hoshi - Journal of Mammalian Ova …, 2004 - jstage.jst.go.jp
Recent advances in technologies associated with assisted reproduction in domestic animals
have been remarkable. In cattle, immature oocytes can be developed to the blastocyst stage …

[HTML][HTML] Some challenges and unrealized opportunities toward widespread use of the in vitro-produced embryo in cattle production

PJ Hansen - animal, 2023 - Elsevier
The embryo produced by in vitro oocyte maturation, fertilization, and embryonic
development is an important resource for genetic improvement and has the potential to …

Development of bovine oocytes matured, fertilized and cultured in a serum-free, chemically defined medium

Y Takagi, K Mori, M Tomizawa, T Takahashi… - Theriogenology, 1991 - Elsevier
This experiment was conducted to determine if bovine embryos derived from in vitro
maturation, fertilization and culture could develop in serum-free medium. Oocytes were …

[PDF][PDF] Development of serum-free culture systems for the ruminant embryo and subsequent assessment of embryo viability

DK Gardner - JOURNAL OF REPRODUCTION AND …, 1999 - biosciproceedings.org
The mammalian embryo undergoes considerable changes in its physiology and energy
metabolism as it proceeds from the zygote to the blastocyst stage. Complete development of …

A single medium supports development of bovine embryos throughout maturation, fertilization and culture

AP Gandhi, M Lane, DK Gardner… - Human …, 2000 - academic.oup.com
Oocytes and embryos are typically exposed sequentially to varying culture media in
standard in-vitro protocols. Expenditures of energy may be required following each medium …