In the first wave of synthetic biology, genetic elements, combined into simple circuits, are
used to control individual cellular functions. In the second wave of synthetic biology, the …

Synthetic circuits offer great promise for generating insights into nature's underlying design
principles or forward engineering novel biotechnology applications. However, construction …

Highlights•We review recent advances in the forward design of genetic circuits.•Advanced
circuit design concepts pave the way for more complex synthetic cellular regulation.•Part …

A major objective of synthetic biology is to make the process of designing genetically
encoded biological systems more systematic, predictable, robust, scalable, and efficient …

Advances in DNA synthesis have enabled the construction of artificial genes, gene circuits,
and genomes of bacterial scale. Freedom in de novo design of synthetic constructs provides …

Engineering biosynthetic pathways for chemical production requires extensive optimization
of the host cellular metabolic machinery. Because it is challenging to specify a priori an …

The ability to engineer biological systems, whether to introduce novel functionality or
improved performance, is a cornerstone of biotechnology and synthetic biology. Typically …

Synthetic biology is focused on the rational construction of biological systems based on
engineering principles. During the field's first decade of development, significant progress …

In the past decade, computational methods have been shown to be well suited to unraveling
the complex web of metabolic reactions in biological systems. Methods based on flux …

Background The field of synthetic biology promises to revolutionize our ability to engineer
biological systems, providing important benefits for a variety of applications. Recent …