Visualizing macromolecules inside bacteria at a high spatial resolution has remained a
challenge owing to their small size and limited resolution of optical microscopy techniques …

Advancements in the field of cryo-electron tomography have greatly contributed to our
current understanding of prokaryotic cell organization and revealed intracellular structures …

Highlights•Electron cryotomography is a powerful in situ imaging technique but restricted to
thin samples.•Cryo-focused ion beam milling allows for sample thinning prior to …

Electron cryotomography (ECT) is an emerging technology that allows thin samples such as
small bacterial cells to be imaged in 3D in a nearly native state to 'molecular'(∼ 4nm) …

Electron cryotomography (cryo-ET) is an imaging technique uniquely suited to the study of
bacterial ultrastructure and cell biology. Recent years have seen a surge in structural and …

Recent advances in three-dimensional electron microscopy provide remarkable tools to
image the interior of bacterial cells. Glimpses of cells at resolutions that are 1–2 orders of …

Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially
native state to'macromolecular'(∼ 4 nm) resolution, revealing the basic architectures of …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

Cryo-electron tomography (cryoET) has become a powerful technique at the interface of
structural biology and cell biology, due to its unique ability for imaging cells in their native …

While much is already known about the basic metabolism of bacterial cells, many
fundamental questions are still surprisingly unanswered, including for instance how they …