A novel method for evaluating and quantifying cell types in platelet rich fibrin and an introduction to horizontal centrifugation

RJ Miron, J Chai, S Zheng, M Feng… - … research Part A, 2019 - Wiley Online Library
Platelet rich fibrin (PRF) has been utilized clinically as a platelet concentrate capable of
stimulating tissue regeneration. Interestingly, several protocols have been proposed with …

A novel method for harvesting concentrated platelet-rich fibrin (C-PRF) with a 10-fold increase in platelet and leukocyte yields

RJ Miron, J Chai, P Zhang, Y Li, Y Wang… - Clinical oral …, 2020 - Springer
Background and objectives Liquid platelet rich fibrin (PRF; often referred to as injectable
PRF) has been utilized as an injectable formulation of PRF that is capable of stimulating …

Histological comparison of Platelet rich fibrin clots prepared by fixed-angle versus horizontal centrifugation

M Fujioka-Kobayashi, M Kono, H Katagiri, B Schaller… - Platelets, 2021 - Taylor & Francis
Platelet-rich fibrin (PRF) is prepared from whole blood without any exogenous coagulation
factors. Several preparation methods have now been introduced, particularly with …

A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: an experimental study in vivo

A Kubesch, M Barbeck, S Al-Maawi, A Orlowska… - Platelets, 2019 - Taylor & Francis
Platelet-rich fibrin (PRF) is generated from the patients' own venous blood by a single
centrifugation step without the additional use of anticoagulants. Based on the previously …

[HTML][HTML] Reduction of the relative centrifugal force influences cell number and growth factor release within injectable PRF-based matrices

S Wend, A Kubesch, A Orlowska, S Al-Maawi… - Journal of Materials …, 2017 - Springer
Platelet rich fibrin (PRF) is a blood concentrate system obtained by centrifugation of
peripheral blood. First PRF matrices exhibited solid fibrin scaffold, more recently liquid PRF …

The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte-and platelet-rich fibrin (L-PRF …

DM Dohan Ehrenfest, NR Pinto, A Pereda, P Jiménez… - Platelets, 2018 - Taylor & Francis
L-PRF (leukocyte-and platelet-rich fibrin) is one of the four families of platelet concentrates
for surgical use and is widely used in oral and maxillofacial regenerative therapies. The first …

[HTML][HTML] Platelet-rich fibrin: Basics of biological actions and protocol modifications

V Pavlovic, M Ciric, V Jovanovic, M Trandafilovic… - Open …, 2021 - degruyter.com
Platelet-rich fibrin (PRF) represents second generation of platelet concentrates, which has
gained increasing awareness in recent years for regenerative procedures. This biologic …

Improved growth factor delivery and cellular activity using concentrated platelet-rich fibrin (C-PRF) when compared with traditional injectable (i-PRF) protocols

M Fujioka-Kobayashi, H Katagiri, M Kono… - Clinical oral …, 2020 - Springer
Objectives Several studies have recently demonstrated that only marginal improvements in
platelet and leukocyte concentrations are achieved following standard injectable platelet …

[HTML][HTML] Impact of g force and timing on the characteristics of platelet-rich fibrin matrices

AB Castro, C Andrade, X Li, N Pinto, W Teughels… - Scientific reports, 2021 - nature.com
Recently, new centrifugation protocols for the preparation of platelet-rich fibrin (PRF) have
been introduced in an attempt to further improve the beneficial impact of these 2nd …

Comparison of platelet-rich fibrin (PRF) produced using 3 commercially available centrifuges at both high (~ 700 g) and low (~ 200 g) relative centrifugation forces

RJ Miron, H Xu, J Chai, J Wang, S Zheng… - Clinical oral …, 2020 - Springer
Objectives Platelet-rich fibrin (PRF) has gained tremendous momentum in recent years as a
natural autologous growth factor derived from blood capable of stimulating tissue …