In situ localization of β‐glucans in the cell wall of Schizosaccharomyces pombe
BM Humbel, M Konomi, T Takagi, N Kamasawa… - Yeast, 2001 - Wiley Online Library
The chemical composition of the cell wall of Sz. pombe is known as β‐1, 3‐glucan, β‐1, 6‐
glucan, α‐1, 3‐glucan and α‐galactomannan; however, the three‐dimensional interactions …
glucan, α‐1, 3‐glucan and α‐galactomannan; however, the three‐dimensional interactions …
A glucanase-driven fractionation allows redefinition of Schizosaccharomyces pombe cell wall composition and structure: assignment of diglucan
PE Magnelli, JF Cipollo, PW Robbins - Analytical biochemistry, 2005 - Elsevier
Purified endoglucanases have been used to determine the composition of
Schizosaccharomyces pombe cell wall. This structure has been traditionally studied after …
Schizosaccharomyces pombe cell wall. This structure has been traditionally studied after …
Analysis and application of a suite of recombinant endo-β(1,3)-d-glucanases for studying fungal cell walls
VSD Carvalho, L Gómez-Delgado, MÁ Curto… - Microbial Cell …, 2021 - Springer
Background The fungal cell wall is an essential and robust external structure that protects
the cell from the environment. It is mainly composed of polysaccharides with different …
the cell from the environment. It is mainly composed of polysaccharides with different …
A refined method for the determination of Saccharomyces cerevisiae cell wall composition and β-1, 6-glucan fine structure
P Magnelli, JF Cipollo, C Abeijon - Analytical biochemistry, 2002 - Elsevier
In yeast and other fungi, cell division, cell shape, and growth depend on the coordinated
synthesis and degradation of cell wall polymers. We have developed a reliable and efficient …
synthesis and degradation of cell wall polymers. We have developed a reliable and efficient …
Mass spectrometric identification of covalently bound cell wall proteins from the fission yeast Schizosaccharomyces pombe
PWJ de Groot, QY Yin, M Weig, GJ Sosinska, FM Klis… - Yeast, 2007 - Wiley Online Library
The cell wall of Schizosaccharomyces pombe is bilayered, consisting of an inner layer of
mainly polysaccharides and an outer layer of galactomannoproteins. We present a detailed …
mainly polysaccharides and an outer layer of galactomannoproteins. We present a detailed …
A combined chemical and enzymatic method to determine quantitatively the polysaccharide components in the cell wall of yeasts
M Schiavone, A Vax, C Formosa… - FEMS yeast …, 2014 - academic.oup.com
A reliable method to determine cell wall polysaccharides composition in yeast is presented,
which combines acid and enzymatic hydrolysis. Sulphuric acid treatment is used to …
which combines acid and enzymatic hydrolysis. Sulphuric acid treatment is used to …
A simple method for quantitative determination of polysaccharides in fungal cell walls
JM Francois - Nature Protocols, 2006 - nature.com
A simple and reliable method for quantitative determination of cell wall polymers in fungal
cell with an sem of 5% is described. This protocol is based on the hydrolysis by sulfuric acid …
cell with an sem of 5% is described. This protocol is based on the hydrolysis by sulfuric acid …
The cell wall of filamentous fungi
This chapter describes the molecular composition and organization of the cell wall in the
filamentous fungi. The major differences between the cell wall of the filamentous fungi and …
filamentous fungi. The major differences between the cell wall of the filamentous fungi and …
Cell Wall Formation in Regenerating Protoplasts of Schizosaccharomyces pombe: Study by High Resolution, Low Voltage Scanning Electron Microscopy
M Osumi, N YAMADA, H KOBORI, A TAKI, N NAITO… - …, 1989 - academic.oup.com
The ultrastructure of regenerating cell wall in Schizosaccharomyces pombe protoplasts was
studied with a high resolution, low voltage scanning electron microscope (LVSEM). In …
studied with a high resolution, low voltage scanning electron microscope (LVSEM). In …