Raman Microspectroscopy and Imaging of Filamentous Fungi

S Shigeto, N Takeshita - Microbes and environments, 2022 - jstage.jst.go.jp
Filamentous fungi grow by the elongation of tubular cells called hyphae and form mycelia
through repeated hyphal tip growth and branching. Since hyphal growth is closely related to …

Exploring morphological and biochemical linkages in fungal growth with label‐free light sheet microscopy and Raman spectroscopy

S Siddhanta, SK Paidi, K Bushley, R Prasad… - …, 2017 - Wiley Online Library
Imaging tip growth in fungal hyphae is highly warranted to unravel the molecular mechanism
of this extraordinarily precise and localized phenomenon. In situ probing of fungal cultures …

Inhomogeneous molecular distributions and cytochrome types and redox states in fungal cells revealed by Raman hyperspectral imaging using multivariate curve …

M Yasuda, N Takeshita, S Shigeto - Analytical chemistry, 2019 - ACS Publications
Hyphae of filamentous fungi consist of compartments that are distinct both spatially and
functionally, thereby forming a unique multicellular system. Much work has been done …

[HTML][HTML] Molecular origin of the Raman signal from Aspergillus nidulans conidia and observation of fluorescence vibrational structure at room temperature

Z Han, BD Strycker, B Commer, K Wang, BD Shaw… - Scientific reports, 2020 - nature.com
Successful approaches to identification and/or biological characterization of fungal
specimens through Raman spectroscopy may require the determination of the molecular …

[HTML][HTML] Label-free chemical imaging of fungal spore walls by Raman microscopy and multivariate curve resolution analysis

H Noothalapati, T Sasaki, T Kaino, M Kawamukai… - Scientific reports, 2016 - nature.com
Fungal cell walls are medically important since they represent a drug target site for
antifungal medication. So far there is no method to directly visualize structurally similar cell …

A sensitive method for examining whole-cell biochemical composition in single cells of filamentous fungi using synchrotron FTIR spectromicroscopy

K Jilkine, KM Gough, R Julian… - Journal of inorganic …, 2008 - Elsevier
Cell function is related to cell composition. The asexual state of filamentous fungi (molds and
mildews) has two main life cycle stages: vegetative hyphae for substrate colonization and …

Utilization of Raman spectroscopy for identification and characterization of fungal pathogens

R Udupa, PP Yegneswaran, J Lukose… - Fungal Biology …, 2023 - Elsevier
Rapid and accurate identification of pathogens causing infections is one of the biggest
challenges of medicine. Timely identification and characterization of fungal pathogens is …

Raman and SERS studies of filamentous fungi

F Farazkhorasani - 2012 - mspace.lib.umanitoba.ca
Fungal species perform many important roles in biotechnology and recycling and act as
agents of disease and decay. Surface-enhanced Raman scattering (SERS) has attracted …

Multimodal molecular imaging and identification of bacterial toxins causing mushroom soft rot and cavity disease

B Dose, T Thongkongkaew, D Zopf, HJ Kim… - …, 2021 - Wiley Online Library
Soft rot disease of edible mushrooms leads to rapid degeneration of fungal tissue and thus
severely affects farming productivity worldwide. The bacterial mushroom pathogen …

A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions

A Szeghalmi, S Kaminskyj, KM Gough - Analytical and bioanalytical …, 2007 - Springer
Synchrotron FTIR can provide high spatial resolution (< 10 μm pixel size) in situ biochemical
analyses of intact biotissues, an area of increasing importance in the post-genomic era, as …