RNA extraction from human cumulus cells (CCs) is considered challenging due to low cell's
pop-ulation and small cell. The successful gene expression analysis from cumulus cells …

Objectives To develop an efficient, economical, and low-toxicity method for the extraction of
RNA from animal cells to meet a basic requirement of biological research: the isolation of …

The extraction of intact RNA from oocyte is quite challenging and time-consuming. A
standard protocol using commercial RNA extraction kit, yields a low quantity of RNA in …

RNA extraction has been improved by integration of a variety of materials in the protocol,
such as phenol, guanidine thiocyanate, and silica, according to the case-specific demands …

RNA isolation is the first step in the study of gene expression and recombinant protein
production. However, the isolation of high quantity and high-quality RNA from tissues …

Background The objectives of this study were to develop an easy and rapid method for
measuring gene expression in a small number of cells by real-time PCR without RNA …

Background Transcriptome analysis during embryogenesis usually requires pooling of
embryos to obtain sufficient RNA. Hence, the measured levels of gene-expression represent …

Gene expression is required for understanding bovine oocytes meiotic maturation as well as
the potential of embryonic development. In the present study a standardized reagent …

Various methods have been described to extract RNA from adherent mammalian cells. RNA
isolation in conjunction with reverse transcription polymerase chain reaction (RT-PCR) is a …

Five RNA extraction methods were tested (CTAB micro extraction, ConcertTM-Invitrogen;
ConcertTM adapted; TRI Reagent®-Sigma; TRI Reagent® adapted) using two different …