Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ

E Kuru, S Tekkam, E Hall, YV Brun… - Nature protocols, 2015 - nature.com
Fluorescent d-amino acids (FDAAs) are efficiently incorporated into the peptidoglycans
(PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and …

In Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with Fluorescent D‐Amino Acids

E Kuru, HV Hughes, PJ Brown, E Hall… - Angewandte …, 2012 - Wiley Online Library
Bacterial growth is controlled by the peptidoglycan (PG) cell wall, a rigid and essential
structure composed of glycan strands cross-linked by d-amino acid (DAA)-containing short …

Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls

YP Hsu, J Rittichier, E Kuru, J Yablonowski… - Chemical …, 2017 - pubs.rsc.org
Fluorescent D-amino acids (FDAAs) enable efficient in situ labeling of peptidoglycan in
diverse bacterial species. Conducted by enzymes involved in peptidoglycan biosynthesis …

d-Amino Acid Derivatives as in Situ Probes for Visualizing Bacterial Peptidoglycan Biosynthesis

YP Hsu, G Booher, A Egan, W Vollmer… - Accounts of chemical …, 2019 - ACS Publications
Conspectus The bacterial cell wall is composed of membrane layers and a rigid yet flexible
scaffold called peptidoglycan (PG). PG provides mechanical strength to enable bacteria to …

Mechanisms of Incorporation for D-Amino Acid Probes That Target Peptidoglycan Biosynthesis

E Kuru, A Radkov, X Meng, A Egan… - ACS Chemical …, 2019 - ACS Publications
Bacteria exhibit a myriad of different morphologies, through the synthesis and modification of
their essential peptidoglycan (PG) cell wall. Our discovery of af luorescent da mino a cid …

Fluorogenic d-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays

YP Hsu, E Hall, G Booher, B Murphy, AD Radkov… - Nature …, 2019 - nature.com
Peptidoglycan is an essential cell wall component that maintains the morphology and
viability of nearly all bacteria. Its biosynthesis requires periplasmic transpeptidation …

Imaging bacterial cell wall biosynthesis

AD Radkov, YP Hsu, G Booher… - Annual review of …, 2018 - annualreviews.org
Peptidoglycan is an essential component of the cell wall that protects bacteria from
environmental stress. A carefully coordinated biosynthesis of peptidoglycan during cell …

Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes

P Shieh, MS Siegrist, AJ Cullen… - Proceedings of the …, 2014 - National Acad Sciences
Fluorescent probes designed for activation by bioorthogonal chemistry have enabled the
visualization of biomolecules in living systems. Such activatable probes with near-infrared …

Bacterial synthesis of d-amino acids

AD Radkov, LA Moe - Applied microbiology and biotechnology, 2014 - Springer
Recent work has shed light on the abundance and diversity of d-amino acids in bacterial
extracellular/periplasmic molecules, bacterial cell culture, and bacteria-rich environments …

Sensing Living Bacteria in Vivo Using d-Alanine-Derived 11C Radiotracers

MFL Parker, JM Luu, B Schulte, TL Huynh… - ACS Central …, 2020 - ACS Publications
Incorporation of d-amino acids into peptidoglycan is a unique metabolic feature of bacteria.
Since d-amino acids are not metabolic substrates in most mammalian tissues, this difference …