An overview of biotransformation for the sustainability of sweet-tasting proteins as natural sugar replacers

EH Yusuf - Chemistry proceedings, 2021 - mdpi.com
According to WHO, sugar intake rates should be reduced due to the connection between
sugar and diseases. However, reducing sugar in foods is a challenge both for food …

Sweet-tasting natural proteins Brazzein and Monellin: safe sugar substitutes for the food industry

TS Novik, EI Koveshnikova, AA Kotlobay, LP Sycheva… - Foods, 2023 - mdpi.com
This article presents the results of a comprehensive toxicity assessment of brazzein and
monellin, yeast-produced recombinant sweet-tasting proteins. Excessive sugar consumption …

Bioprospecting and biotechnological insights into sweet-tasting proteins by microbial hosts―a review

M Bilal, L Ji, S Xu, Y Zhang, HMN Iqbal, H Cheng - Bioengineered, 2022 - Taylor & Francis
Owing to various undesirable health effects of sugar overconsumption, joint efforts are being
made by industrial sectors and regulatory authorities to reduce sugar consumption practices …

Sweet protein brazzein as a promising sweetener

EV Markova, EI Leonova, JV Sopova - Voprosy Pitaniia, 2024 - europepmc.org
The excessive consumption of sugar-containing foods contributes to the development of a
number of diseases, including obesity, diabetes mellitus, etc. As a substitute for sugar …

[HTML][HTML] Application of sweet and taste modifying genes for development in plants: current status and prospects

S Akter, MA Huq, YJ Jung, YG Cho… - Journal of Plant …, 2016 - kspbtjpb.org
Sweet and taste modifying proteins are natural alternatives to synthetic sweeteners and
flavor enhancers, and have been used for centuries in different countries. Use of these …

An updated multifaceted overview of sweet proteins and dipeptides as sugar substitutes; the chemistry, health benefits, gut interactions, and safety

MA Farag, MM Rezk, MH Elashal, M El-Araby… - Food Research …, 2022 - Elsevier
Artificial sweeteners have become increasingly popular worldwide owing to their lower
calorie content in addition to the claims of health benefits such as weight control, blood …

Removal of the N‐terminal methionine improves the sweetness of the recombinant expressed sweet‐tasting protein brazzein and its mutants in Escherichia coli

B Liu, H Jiang, H Wang, L Yang - Journal of Food Biochemistry, 2021 - Wiley Online Library
The sweet‐tasting protein brazzein is the smallest sweet protein with high sweet potency.
Overexpression of this protein in a heterogenous host is an essential way for its production …

Developments in biotechnological production of sweet proteins

T Masuda, N Kitabatake - Journal of bioscience and bioengineering, 2006 - Elsevier
Most proteins are tasteless and flavorless, while some proteins elicit a sweet-taste response
on the human palate. Six proteins, thaumatin, monellin, mabinlin, brazzein, egg lysozyme …

Synthesis and production of sweet-tasting protein in E. coli and purification by amylose resin

F Mansouri, MH MODARESI, M Abolhassani, K Parivar - 2011 - sid.ir
A sweet water-soluble protein that reminds stable over wide ranges of temperature and pH,
BRAZZEIN has various applications. Its tastes like cane sugar but have no calories …

Improved expression of recombinant sweet-tasting brazzein using codon optimization and host change as new strategies

V Jafarian, K Bagheri, J Zarei, S Karami… - Food …, 2020 - Taylor & Francis
There is increased food industry attention to low-calorie natural sweeteners with good taste
properties such as in plant-based sweet proteins. Brazzein is an attractive alternative to …