Optimasi ekspresi human Epidermal Growth Factor (h-EGF) rekombinan dalam Escherichia coli BL21 (DE3) dengan variasi media dan konsentrasi penginduksi

RD Pratiwi - Chimica et Natura Acta, 2019 - ijcp.or.id
Epidermal growth factor (EGF) merupakan protein yang sangat penting dalam proliferasi,
diferensiasi, dan pematangan sel. EGF sintetik atau recombinant human EGF (rh-EGF) telah …

[PDF][PDF] Modification of recombinant human epidermal growth factor (rh-EGF) expression vector by site-directed mutagenesis for therapeutic protein production

A Rodiansyah, RD Pratiwi, S Zanjabila… - Indonesian Journal of …, 2019 - researchgate.net
Recombinant human epidermal growth factor (rh-EGF) has high value in therapies for h-EGF
deficiency-related diseases. The expression of the h-EGF gene was designed by using the …

An approach for recombinant epidermal growth factor purification using an elastin-like protein tag

R Sarvestani, AM Latifi, H Alizadeh… - Journal of Applied …, 2021 - eprints.bmsu.ac.ir
Introduction: Wide applications in research, clinical and cosmetic industry of human
epidermal growth factor (hEGF) has made it as a research interest target. Its production in …

Expression of Recombinant Human Epidermal Growth Factor in Escherichia coli and Characterization of its Biological Activity

AF Abdull Razis, EN Ismail, Z Hambali… - Applied biochemistry …, 2008 - Springer
Recombinant human epidermal growth factor (EGF) was successfully expressed as a fusion
protein in Escherichia coli system. This system was used OmpA signal sequence to produce …

Economic optimization of expression of soluble human epidermal growth factor in Escherichia coli

K Liu, FQ Wang, M Zhao, B Gao, H Xu, D Wei - Biotechnology Letters, 2022 - Springer
Human epidermal growth factor (hEGF) has multiple biological functions, such as promoting
cell proliferation, differentiation, and migration. In addition, it is a very expensive polypeptide …

Co-expression of recombinant human epidermal growth factor (rhEGF) In Escherichia coli BL21 (DE3) with Bacillus cereus phospholipase C

A Indriyani, S Gaffar, FPU Latifah… - AIP Conference …, 2020 - pubs.aip.org
Diabetes mellitus is a metabolic disorder in the body caused by high levels of blood sugar.
Diabetic foot ulcer is a further complication due to ulceration in the feet of diabetic patients …

Rapid Screening for High Expressing Multicopy Recombinants and Enhanced Epidermal Growth Factor (EGF) Protein Production Using Pichia Pastoris

Z Guo, Z Li, L Li, Q Zhao, W Li, A Diao - International Journal of Peptide …, 2022 - Springer
Epidermal growth factor (EGF) is a highly effective stimulator which promotes cell
proliferation and this has led it to being proposed as a prospective healing agent for various …

Study on Soluble Expression of Recombinant Human Epidermal Growth Factor Using Various Fusion Partners in Escherichia coli

BL Kim, JE Baek, EG Lee, HW Lee, CS Kim… - 한국생물공학회학술 …, 2007 - earticle.net
The efficient soluble expression of human epidermal growth factor (hEGF) was achieved by
using functional fusion partners in cytoplasm and periplasm of Escherichia coli (E. coli) …

[HTML][HTML] Construction of yeast recombinant expression vector containing human epidermal growth factor (hEGF)

J Mohammadian, S Mansoori-Derakhshan… - Advanced …, 2013 - ncbi.nlm.nih.gov
Purpose: The objective of this study was construction of recombinant hEGF-pPIC9 which
may be used for expression of recombinant hEGF in following studies. Methods: EGF cDNA …

Expression of Recombinant Human Epidermal Growth Factor as a Active Form through Codon Optimization with E. coli and Co-expression of Chaperone

EB Jang, JS Kim, WY Lee - Journal of the Korea Academia …, 2020 - koreascience.kr
Epidermal growth factor (EGF) is a hormone protein that affects cell growth and proliferation,
and has various medical applications. In the present study, the gene of human EGF was …